Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …

Recombinational DNA repair is a universal aspect of DNA metabolism and is essential for
genomic integrity. It is a template-directed process that uses a second chromosomal copy …

In nanopore analytics, individual molecules pass through a single nanopore giving rise to
detectable temporary blockades in ionic pore current. Reflecting its simplicity, nanopore …

This review provides an outline for fluorescent labeling of proteins. Fluorescent assays are
very diverse providing the most sensitive and robust methods for observing biological …

It has been over 20 years since the pioneering work of Arthur Ashkin, and in the intervening
years, the field of optical tweezers has grown tremendously. Optical tweezers are now being …

The basic features of DNA were elucidated during the half-century following the discovery of
the double helix. But it is only during the past decade that researchers have been able to …

The ability to obtain a molecular-level understanding of the chemistry that takes place at
liquid/solid interfaces is key to the development and improvement of many chemical and …

SUMMARY The RecBCD enzyme of Escherichia coli is a helicase-nuclease that initiates the
repair of double-stranded DNA breaks by homologous recombination. It also degrades …

DNA intercalators are widely used as fluorescent probes to visualize DNA and DNA
transactions in vivo and in vitro. It is well known that they perturb DNA structure and stability …

Helicases are motor proteins that couple conformational changes induced by ATP binding
and hydrolysis with unwinding of duplex nucleic acid,,, and are involved in several human …