Microfluidic techniques for high throughput single cell analysis
Highlights•Microfluidics complements high sensitivity molecular tools, allowing single cell
analysis.•Cells can be directly manipulated by a variety of fields, with forces equivalent …
analysis.•Cells can be directly manipulated by a variety of fields, with forces equivalent …
Three-dimensional models for studying development and disease: moving on from organisms to organs-on-a-chip and organoids
EL Jackson, H Lu - Integrative Biology, 2016 - academic.oup.com
Human development and disease are challenging to study because of lack of experimental
accessibility to in vivo systems and the complex nature of biological processes. For these …
accessibility to in vivo systems and the complex nature of biological processes. For these …
A review on microfluidics manipulation of the extracellular chemical microenvironment and its emerging application to cell analysis
Spatiotemporal manipulation of extracellular chemical environments with simultaneous
monitoring of cellular responses plays an essential role in exploring fundamental biological …
monitoring of cellular responses plays an essential role in exploring fundamental biological …
Fluorescent analysis of bioactive molecules in single cells based on microfluidic chips
Y Fan, D Dong, Q Li, H Si, H Pei, L Li, B Tang - Lab on a Chip, 2018 - pubs.rsc.org
Single-cell analysis of bioactive molecules is an essential strategy for a better understanding
of cell biology, exploring cell heterogeneity, and improvement of the ability to detect early …
of cell biology, exploring cell heterogeneity, and improvement of the ability to detect early …
Improved cell adhesion under shear stress in PDMS microfluidic devices
Microfluidic systems based on polydimethylsiloxane (PDMS) provide a versatile platform to
study the mechanoresponse of cells in vitro. Under a shear flow, however, the stability of …
study the mechanoresponse of cells in vitro. Under a shear flow, however, the stability of …
A sharp-edge-based acoustofluidic chemical signal generator
Resolving the temporal dynamics of cell signaling pathways is essential for regulating
numerous downstream functions, from gene expression to cellular responses. Mapping …
numerous downstream functions, from gene expression to cellular responses. Mapping …
Multichannel synchronous hydrodynamic gating coupling with concentration gradient generator for high-throughput probing dynamic signaling of single cells
Y Guo, Z Gao, Y Liu, S Li, J Zhu, P Chen… - Analytical …, 2020 - ACS Publications
Cell signaling greatly affected by complicated and temporally dynamic extracellular
microenvironments controls most of the physiological functions in vivo. To reconstruct or …
microenvironments controls most of the physiological functions in vivo. To reconstruct or …
An automated programmable platform enabling multiplex dynamic stimuli delivery and cellular response monitoring for high-throughput suspension single-cell …
Cell signaling events are orchestrated by dynamic external biochemical cues. By rapidly
perturbing cells with dynamic inputs and examining the output from these systems, one …
perturbing cells with dynamic inputs and examining the output from these systems, one …
Compartmentalized microchannel array for high-throughput analysis of single cell polarized growth and dynamics
T Geng, EL Bredeweg, CJ Szymanski, B Liu… - Scientific Reports, 2015 - nature.com
Interrogating polarized growth is technologically challenging due to extensive cellular
branching and uncontrollable environmental conditions in conventional assays. Here we …
branching and uncontrollable environmental conditions in conventional assays. Here we …
Review of microfluidic cell culture devices for the control of gaseous microenvironments in vitro
Gaseous microenvironments play important roles in various biological activities in vivo.
However, it is challenging to precisely control gaseous microenvironments in vitro for cell …
However, it is challenging to precisely control gaseous microenvironments in vitro for cell …