This review commemorates the 40th anniversary of DNA sequencing, a period in which we
have already witnessed multiple technological revolutions and a growth in scale from a few …

The term next-generation sequencing is almost a decade old, but it remains the colloquial
way to describe highly parallel or high-output sequencing methods that produce data at or …

An effective blood-based method for the diagnosis and prognosis of hepatocellular
carcinoma (HCC) has not yet been developed. Circulating tumour DNA (ctDNA) carrying …

Circulating tumor DNA (ctDNA) has emerged as a useful diagnostic and prognostic
biomarker in many cancers. Here, we conducted a study to investigate the potential use of …

The ability to identify a specific cancer using minimally invasive biopsy holds great promise
for improving the diagnosis, treatment selection, and prediction of prognosis in cancer …

Crop production needs to increase to secure future food supplies, while reducing its impact
on ecosystems. Detailed characterization of plant genomes and genetic diversity is crucial …

For over 60 years, the synthetic production of new DNA sequences has helped researchers
understand and engineer biology. Here we summarize methods and caveats for the de novo …

Abstract RNA-sequencing (RNA-seq) measures the quantitative change in gene expression
over the whole transcriptome, but it lacks spatial context. In contrast, in situ hybridization …

高通量测序技术是DNA 测序发展历程的一个里程碑, 它为现代生命科学研究提供了前所未有的
机遇. 详细介绍了以454, Solexa 和SOLiD 为代表的第二代高通量测序技术, 以HeliScope TIRM …

Exome sequencing studies of autism spectrum disorders (ASDs) have identified many de
novo mutations but few recurrently disrupted genes. We therefore developed a modified …