When used appropriately, a confocal fluorescence microscope is an excellent tool for
making quantitative measurements in cells and tissues. The confocal microscope's ability to …

Phototoxicity frequently occurs during live fluorescence microscopy, and its consequences
are often underestimated. Damage to cellular macromolecules upon excitation light …

Although fluorescence microscopy is ubiquitous in biomedical research, microscopy
methods reporting is inconsistent and perhaps undervalued. We emphasize the importance …

Multiple scattering limits the contrast in optical imaging of thick specimens. Here, we present
gradient light interference microscopy (GLIM) to extract three-dimensional information from …

Fluorescence microscopy has become a ubiquitous method to observe the location of
specific molecular components within cells. However, the resolution of light microscopy is …

The extracellular matrix (ECM) microenvironment plays a central role in cell migration by
providing physiochemical information that influences overall cell behavior. Much of this …

Despite recent improvements in microscope technologies, segmenting and tracking cells in
three-dimensional time-lapse images (3D+ T images) to extract their dynamic positions and …

Biological responses to ionizing radiation (IR) have been studied for many years, generally
showing the dependence of these responses on the quality of radiation, ie, the radiation …

Modern microscopes used for biological imaging often present themselves as black boxes
whose precise operating principle remains unknown, and whose optical resolution and price …

The confocal fluorescence microscope has become a popular tool for life sciences
researchers, primarily because of its ability to remove blur from outside of the focal plane of …