Thiopeptides (also known as thiazolyl peptides) are structurally complex natural products
with rich biological activities. Known for over 70 years for potent killing of Gram-positive …

Bioengineering of ribosomally synthesized and post-translationally modified peptides
(RiPPs) is an emerging approach to explore the diversity of pseudo-natural product …

Broad substrate tolerance of ribosomally synthesized and post-translationally modified
peptide (RiPP) biosynthetic enzymes has allowed numerous strategies for RiPP …

Since the start of the 21st century, antibiotic drug discovery and development from natural
products has experienced a certain renaissance. Currently, basic scientific research in …

Lactazole A is a cryptic thiopeptide from Streptomyces lactacystinaeus, encoded by a
compact 9.8 kb biosynthetic gene cluster. Here, we establish a platform for in vitro …

Thiopeptides make up a group of structurally complex peptidic natural products holding
promise in bioengineering applications. The previously established thiopeptide/mRNA …

S‐Adenosylmethionine (SAM) is one of the most common co‐substrates in enzyme‐
catalyzed methylation reactions. Most SAM‐dependent reactions proceed through an SN2 …

Nosiheptide (NOS), a potent bactericidal thiopeptide, belongs to a class of natural products
produced by ribosomal synthesis and post-translational modifications, and its biosynthetic …

Nosiheptide is a prototypal thiopeptide antibiotic, containing an indole side ring in addition
to its thiopeptide-characteristic macrocylic scaffold. This indole ring is derived from 3-methyl …

NosN is a class C radical S-adenosylmethionine (SAM) methyltransferase (RSMT) involved
in the biosynthesis of nosiheptide, a clinically interesting thiopeptide antibiotic produced by …