The CRISPR–Cas toolbox for analytical and diagnostic assay development
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
[HTML][HTML] CRISPR-Cas12a: Functional overview and applications
B Paul, G Montoya - Biomedical journal, 2020 - Elsevier
Prokaryotes have developed an adaptive immune system called Clustered regularly
interspaced short palindromic repeats (CRISPR) to combat attacks by foreign mobile genetic …
interspaced short palindromic repeats (CRISPR) to combat attacks by foreign mobile genetic …
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs
The use of microRNAs as clinical cancer biomarkers is hindered by the absence of accurate,
fast and inexpensive assays for their detection in biofluids. Here we report a one-step and …
fast and inexpensive assays for their detection in biofluids. Here we report a one-step and …
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
JS Chen, E Ma, LB Harrington, M Da Costa, X Tian… - Science, 2018 - science.org
CRISPR-Cas12a (Cpf1) proteins are RNA-guided enzymes that bind and cut DNA as
components of bacterial adaptive immune systems. Like CRISPR-Cas9, Cas12a has been …
components of bacterial adaptive immune systems. Like CRISPR-Cas9, Cas12a has been …
Boosting plant genome editing with a versatile CRISPR-Combo system
CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided
genome engineering in plants. However, these systems are mostly used separately, leaving …
genome engineering in plants. However, these systems are mostly used separately, leaving …
Mechanistic Insights into the cis-and trans-Acting DNase Activities of Cas12a
Summary CRISPR-Cas12a (Cpf1) is an RNA-guided DNA-cutting nuclease that has been
repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target …
repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target …
Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts
The ability to modify multiple genetic elements simultaneously would help to elucidate and
control the gene interactions and networks underlying complex cellular functions. However …
control the gene interactions and networks underlying complex cellular functions. However …
Molecular mechanisms of CRISPR-Cas immunity in bacteria
PM Nussenzweig, LA Marraffini - Annual review of genetics, 2020 - annualreviews.org
Prokaryotes have developed numerous defense strategies to combat the constant threat
posed by the diverse genetic parasites that endanger them. Clustered regularly interspaced …
posed by the diverse genetic parasites that endanger them. Clustered regularly interspaced …
Kinetic basis for DNA target specificity of CRISPR-Cas12a
Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising
applications in human health and disease. However, DNA cleavage at off-target sites that …
applications in human health and disease. However, DNA cleavage at off-target sites that …
CRISPR enzyme kinetics for molecular diagnostics
A Ramachandran, JG Santiago - Analytical chemistry, 2021 - ACS Publications
CRISPR-diagnostic assays have gained significant interest in the last few years. This
interest has grown rapidly during the current COVID-19 pandemic, where CRISPR …
interest has grown rapidly during the current COVID-19 pandemic, where CRISPR …