Observation of molecular processes inside living cells is fundamental to a quantitative
understanding of how biological systems function. Specifically, decoding the complex …

Methods for imaging and tracking single molecules conjugated with fluorescent probes,
called single-molecule tracking (SMT), are now providing researchers with the …

Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence
microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes …

Counting molecules in complexes is challenging, even with super-resolution microscopy.
Here, we use the programmable and specific binding of dye-labeled DNA probes to count …

The genomes of multicellular organisms are extensively folded into 3D chromosome
territories within the nucleus. Advanced 3D genome-mapping methods that combine …

Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence
microscope without requiring high laser powers or specialized fluorophores. However, the …

Protein clustering is a hallmark of genome regulation in mammalian cells. However, the
dynamic molecular processes involved make it difficult to correlate clustering with functional …

Histone modifications influence higher-order chromatin structures at individual epigenomic
states and chromatin environments to regulate gene expression. However, genome-wide …

The assembly of sequence-specific enhancer-binding transcription factors (TFs) at cis-
regulatory elements in the genome has long been regarded as the fundamental mechanism …

The three-dimensional packing of the genome plays an important role in regulating gene
expression. We have used Hi-C, a genome-wide chromatin conformation capture (3C) …