Background The processes of DNA supercoiling and transcription are interdependent
because the movement of a transcription elongation complex simultaneously induces under …

DNA supercoiling controls a variety of cellular processes, including transcription,
recombination, chromosome replication, and segregation, across all domains of life. As a …

Understanding cellular life requires a comprehensive knowledge of the essential cellular
functions, the components involved, and their interactions. Minimized genomes are an …

CRISPR-Cas9 is a powerful gene-editing technology; however, off-target activity remains an
important consideration for therapeutic applications. We have previously shown that force …

Supercoiling impacts DNA replication, transcription, protein binding to DNA, and the three-
dimensional organization of chromosomes. However, there are currently no methods to …

Two enzymes are responsible for maintaining supercoiling in the human pathogen
Streptococcus pneumoniae, gyrase (GyrA2GyrB2) and topoisomerase I. To attain diverse …

DNA supercoiling acts as a global and ancestral regulator of bacterial gene expression. In
this review, we advocate that it plays a pivotal role in host-pathogen interactions by …

Early studies of transcriptional regulation focused on individual gene promoters defined
specific transcription factors as central agents of genetic control. However, recent genome …

Chromosomes are dynamic entities, whose organization and structure depend on the
concerted activity of DNA-binding proteins and DNA-processing enzymes. In bacteria …

The histone-like protein HU is a conserved nucleoid-associated protein that is involved in
the maintenance of the bacterial chromosome architecture. It is the only known nucleoid …