Contemporary hydrogen deuterium exchange mass spectrometry

I Oganesyan, C Lento, DJ Wilson - Methods, 2018 - Elsevier
Hydrogen/deuterium exchange (HDX) mass spectrometry (MS) emerged as a tool for
biochemistry and structural biology around 25 years ago. It has since become a key …

Limitations and pitfalls in protein identification by mass spectrometry

G Lubec, L Afjehi-Sadat - Chemical reviews, 2007 - ACS Publications
The advent of proteomics techniques for protein identification (PrI) represented a major step
forward in protein chemistry, 1, 2 and indeed, a legion of laboratories are now using different …

ExPASy: the proteomics server for in-depth protein knowledge and analysis

E Gasteiger, A Gattiker, C Hoogland… - Nucleic acids …, 2003 - academic.oup.com
Abstract The ExPASy (the Expert Protein Analysis System) World Wide Web server
(http://www. expasy. org), is provided as a service to the life science community by a …

Mining a tandem mass spectrometry database to determine the trends and global factors influencing peptide fragmentation

EA Kapp, F Schütz, GE Reid, JS Eddes… - Analytical …, 2003 - ACS Publications
A database of 5500 unique peptide tandem mass spectra acquired in an ion trap mass
spectrometer was assembled for peptides derived from proteins digested with trypsin …

Prediction of missed cleavage sites in tryptic peptides aids protein identification in proteomics

JA Siepen, EJ Keevil, D Knight… - Journal of proteome …, 2007 - ACS Publications
Protein identification via peptide mass fingerprinting (PMF) remains a key component of high-
throughput proteomics experiments in post-genomic science. Candidate protein …

Site-Specific N-Linked Glycosylation Analysis of Human Carcinoembryonic Antigen by Sheathless Capillary Electrophoresis–Tandem Mass Spectrometry

L Pont, V Kuzyk, F Benavente… - Journal of proteome …, 2021 - ACS Publications
With 28 potential N-glycosylation sites, human carcinoembryonic antigen (CEA) bears an
extreme amount of N-linked glycosylation, and approximately 60% of its molecular mass can …

Mining the structural genomics pipeline: identification of protein properties that affect high-throughput experimental analysis

CS Goh, N Lan, SM Douglas, B Wu, N Echols… - Journal of molecular …, 2004 - Elsevier
Structural genomics projects represent major undertakings that will change our
understanding of proteins. They generate unique datasets that, for the first time, present a …

Interlaboratory study on differential analysis of protein glycosylation by mass spectrometry: the ABRF glycoprotein research multi-institutional study 2012

N Leymarie, PJ Griffin, K Jonscher, D Kolarich… - Molecular & Cellular …, 2013 - ASBMB
One of the principal goals of glycoprotein research is to correlate glycan structure and
function. Such correlation is necessary in order for one to understand the mechanisms …

Proteomic analysis of κ‐casein micro‐heterogeneity

JW Holland, HC Deeth, PF Alewood - Proteomics, 2004 - Wiley Online Library
The proteome of bovine milk is dominated by just six gene products that constitute
approximately 95% of milk protein. Nonetheless, over 150 protein spots can be readily …

Annotation of post‐translational modifications in the Swiss‐Prot knowledge base

N Farriol‐Mathis, JS Garavelli, B Boeckmann… - …, 2004 - Wiley Online Library
High‐throughput proteomic studies produce a wealth of new information regarding post‐
translational modifications (PTMs). The Swiss‐Prot knowledge base is faced with the …