Hydrogen peroxide stress provokes a metabolic reprogramming in Pseudomonas fluorescens: enhanced production of pyruvate

A Bignucolo, VP Appanna, SC Thomas, C Auger… - Journal of …, 2013 - Elsevier
Pseudomonas fluorescens invoked a metabolic reconfiguration that resulted in enhanced
production of pyruvate under the challenge of hydrogen peroxide (H 2 O 2). Although this …

Zinc toxicity and ATP production in Pseudomonas fluorescens

A Alhasawi, C Auger, VP Appanna… - Journal of applied …, 2014 - academic.oup.com
Aims To identify the molecular networks in Pseudomonas fluorescens that convey resistance
to toxic concentrations of Zn, a common pollutant and hazard to biological systems. Methods …

The unravelling of metabolic dysfunctions linked to metal-associated diseases by blue native polyacrylamide gel electrophoresis

S Han, C Auger, Z Castonguay, VP Appanna… - Analytical and …, 2013 - Springer
Gel electrophoresis is routinely used to separate and analyse macromolecules in biological
systems. Although many of these electrophoretic techniques necessitate the denaturing of …

A novel ATP-generating machinery to counter nitrosative stress is mediated by substrate-level phosphorylation

C Auger, VD Appanna - Biochimica et Biophysica Acta (BBA)-General …, 2015 - Elsevier
Background It is well-known that elevated amounts of nitric oxide and other reactive nitrogen
species (RNS) impact negatively on the tricarboxylic acid (TCA) cycle and oxidative …

[HTML][HTML] Deciphering metabolic networks by blue native polyacrylamide gel electrophoresis: a functional proteomic exploration

C Auger, ND Appanna, A Alhasawi, VD Appanna - EuPA Open Proteomics, 2015 - Elsevier
Metabolism is the consortium of reactions within a cell which directs a variety of processes
including energy synthesis, signalling and the behaviour of a biological system. Metabolic …

Metabolic adaptation and ATP homeostasis in Pseudomonas fluorescens exposed to phosphate stress

F Legendre, A MacLean, S Tharmalingam… - World Journal of …, 2022 - Springer
Phosphate (Pi) is essential for life as it is an integral part of the universal chemical energy
adenosine triphosphate (ATP), and macromolecules such as, DNA, RNA proteins and lipids …

Aspartate metabolism and pyruvate homeostasis triggered by oxidative stress in Pseudomonas fluorescens: a functional metabolomic study

A Alhasawi, M Leblanc, ND Appanna, C Auger… - Metabolomics, 2015 - Springer
There is mounting evidence that metabolic reprogramming is critical for the survival of
organisms exposed to changing and stressed environments. Using the soil microbe …

Metabolic reconfigurations aimed at the detoxification of a multi-metal stress in Pseudomonas fluorescens: Implications for the bioremediation of metal pollutants

A Alhasawi, J Costanzi, C Auger, ND Appanna… - Journal of …, 2015 - Elsevier
Although the ability of microbial systems to adapt to the toxic challenge posed by numerous
metal pollutants individually has been well documented, there is little detailed information on …

Metabolic networks to generate pyruvate, PEP and ATP from glycerol in Pseudomonas fluorescens

A Alhasawi, SC Thomas, VD Appanna - Enzyme and Microbial Technology, 2016 - Elsevier
Glycerol is a major by-product of the biodiesel industry. In this study we report on the
metabolic networks involved in its transformation into pyruvate, phosphoenolpyruvate (PEP) …

A blue native polyacrylamide gel electrophoretic technology to probe the functional proteomics mediating nitrogen homeostasis in Pseudomonas fluorescens

S Han, C Auger, VP Appanna, J Lemire… - Journal of …, 2012 - Elsevier
As glutamate and ammonia play a pivotal role in nitrogen homeostasis, their production is
mediated by various enzymes that are widespread in living organisms. Here, we report on …