Based on engineered or bacterial nucleases, the development of genome editing
technologies has opened up the possibility of directly targeting and modifying genomic …

Non-homologous DNA end joining (NHEJ) is the predominant repair mechanism of any type
of DNA double-strand break (DSB) during most of the cell cycle and is essential for the …

Microbial systems underpin many biotechnologies, including CRISPR, but the exponential
growth of sequence databases makes it difficult to find previously unidentified systems. In …

In vertebrate cells, the DNA damage response is controlled by three related kinases: ATM,
ATR, and DNA-PK. It has been 20 years since the cloning of ATR, the last of the three to be …

DNA double-strand breaks (DSBs) are the most dangerous type of DNA damage because
they can result in the loss of large chromosomal regions. In all mammalian cells, DSBs that …

Molecular, cellular, and clinical studies of human inborn errors of immunity have
revolutionized our understanding of their pathogenesis, considerably broadened their …

Nonhomologous DNA end-joining (NHEJ) is the predominant double-strand break (DSB)
repair pathway throughout the cell cycle and accounts for nearly all DSB repair outside of …

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9
(Cas9) is a precise genome manipulating technology that can be programmed to induce …

DNA damage created by endogenous or exogenous genotoxic agents can exist in multiple
forms, and if allowed to persist, can promote genome instability and directly lead to various …

Microarrays can measure the expression of thousands of genes to identify changes in
expression between different biological states. Methods are needed to determine the …