A century of enzyme kinetics. Should we believe in the Km and vmax estimates?

S Schnell, PK Maini - 2003 - ora.ox.ac.uk
The application of the quasi-steady-state approximation (QSSA) in biochemical kinetics
allows the reduction of a complex biochemical system with an initial fast transient into a …

Monitoring protein conformational changes using fluorescent nanoantennas

SG Harroun, D Lauzon, MC Ebert, A Desrosiers… - Nature …, 2022 - nature.com
Understanding the relationship between protein structural dynamics and function is crucial
for both basic research and biotechnology. However, methods for studying the fast dynamics …

Optimal methods for estimating kinetic isotope effects from different forms of the Rayleigh distillation equation

KM Scott, X Lu, CM Cavanaugh, JS Liu - Geochimica et Cosmochimica Acta, 2004 - Elsevier
The interpretation of stable isotope values hinges on precise, accurate estimates of kinetic
isotope effects (α), which are equal to 1k/2k, where 1k and 2k are the reaction rate constants …

Fitting light saturation curves measured using modulated fluorometry

RJ Ritchie - Photosynthesis Research, 2008 - Springer
Abstract A blue diode PAM (P ulse A mplitude M odulation) fluorometer was used to
measure rapid Photosynthesis (P) versus Irradiance (E) curves (P vs. E curves) in …

Uncertainty Quantification of Michaelis–Menten Kinetic Rates and Its Application to the Analysis of CRISPR‐Based Diagnostics

AS Avaro, JG Santiago - Angewandte Chemie, 2022 - Wiley Online Library
Michaelis–Menten kinetics is an essential model to rationalize enzyme reactions. The
quantification of Michaelis–Menten parameters can be very challenging as it is sensitive to …

On the estimation errors of KM and V from time-course experiments using the Michaelis–Menten equation

W Stroberg, S Schnell - Biophysical chemistry, 2016 - Elsevier
The conditions under which the Michaelis–Menten equation accurately captures the steady-
state kinetics of a simple enzyme-catalyzed reaction is contrasted with the conditions under …

Supplementation of cooked broccoli with exogenous moringa myrosinase enhanced isothiocyanate formation

Y Zhang, N Makaza, C Jiang, Y Wu, SZ Nishanbaev… - Food Chemistry, 2022 - Elsevier
Brassica vegetables, especially broccoli, have health benefits such as anticancer activity,
which are attributed to isothiocyanate (ITC), products of glucosinolate hydrolysis. This study …

[HTML][HTML] Purification and characteristics of a novel milk-clotting metalloprotease from Bacillus velezensis DB219

Y Zhang, J Hu, J Wang, C Liu, X Liu, J Sun… - Journal of Dairy …, 2023 - Elsevier
Milk-clotting enzyme (MCE) is the essential active agents in dairy processing. The traditional
MCE is mainly obtained from animal sources, in which calf rennet is the most widely used in …

Culture of Spirulina platensis in human urine for biomass production and O2 evolution

D Feng, Z Wu - Journal of Zhejiang University Science B, 2006 - Springer
Attempts were made to culture Spirulina platensis in human urine directly to achieve
biomass production and O 2 evolution, for potential application to nutrient regeneration and …

Uptake kinetics and assimilation of inorganic nitrogen by Catenella nipae and Ulva lactuca

JW Runcie, RJ Ritchie, AWD Larkum - Aquatic Botany, 2003 - Elsevier
The kinetics of NH4+, the assimilation of NH4+ and nitrate uptake by Catenella nipae
(Rhodophyta) were compared with Ulva lactuca (Chlorophyta). Both algal species …