This review provides a state-of-the-art description of the performance of Sanger cycle
sequencing of the 16S rRNA gene for routine identification of bacteria in the clinical …

Lipids comprise a large group of chemically heterogeneous compounds. The majority have
fatty acids (FA) as part of their structure, making these compounds suitable tools to examine …

The rapid development of next-generation sequencing (NGS) technology, including
advances in sequencing chemistry, sequencing technologies, bioinformatics, and data …

Bacterial culture was the first method used to describe the human microbiota, but this
method is considered outdated by many researchers. Metagenomics studies have since …

The use of 16S rRNA gene sequences to study bacterial phylogeny and taxonomy has been
by far the most common housekeeping genetic marker used for a number of reasons. These …

The traditional identification of bacteria on the basis of phenotypic characteristics is
generally not as accurate as identification based on genotypic methods. Comparison of the …

In the last decade, as a result of the widespread use of PCR and DNA sequencing, 16S
rDNA sequencing has played a pivotal role in the accurate identification of bacterial isolates …

Some bacteria are difficult to identify with phenotypic identification schemes commonly used
outside reference laboratories. 16S ribosomal DNA (rDNA)-based identification of bacteria …

Endophytic bacteria reside within plant hosts without causing disease symptoms. In this
study, 853 endophytic strains were isolated from aerial tissues of four agronomic crop …

Three hundred and twenty isolates of Staphylococcus aureus were typed by DNA sequence
analysis of the X region of the protein A gene (spa). spa typing was compared to both …