Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies
S Sahdev, SK Khattar, KS Saini - Molecular and cellular biochemistry, 2008 - Springer
Among the various expression systems employed for the over-production of proteins,
bacteria still remains the favorite choice of a Protein Biochemist. However, even today, due …
bacteria still remains the favorite choice of a Protein Biochemist. However, even today, due …
Escherichia coli one-and two-hybrid systems for the analysis and identification of protein–protein interactions
JC Hu, MG Kornacker, A Hochschild - Methods, 2000 - Elsevier
Genetic methods based on fusion proteins allow the power of a genetic approach to be
applied to the self-assembly of proteins or protein fragments, regardless of whether or not …
applied to the self-assembly of proteins or protein fragments, regardless of whether or not …
[PDF][PDF] Protein-protein interaction between Bacillus stearothermophilus tyrosyl-tRNA synthetase subdomains revealed by a bacterial two-hybrid system
G Karimova, A Ullmann, D Ladant - Journal of molecular microbiology …, 2001 - caister.com
We have recently developed a bacterial two-hybrid system (BACTH), based on functional
complementation between two fragments of the catalytic domain of Bordetella pertussis …
complementation between two fragments of the catalytic domain of Bordetella pertussis …
Evolutionary approaches to protein engineering
B Steipe - Combinatorial Chemistry in Biology, 1999 - Springer
The very term “protein engineering” remains something of an oxymoron, at least as far as
engineering implies the rational application of well understood principles towards achieving …
engineering implies the rational application of well understood principles towards achieving …
[HTML][HTML] Genetic systems for analyzing protein–protein interactions in bacteria
D Ladant, G Karimova - Research in microbiology, 2000 - Elsevier
Analysis of protein–protein interactions has been revolutionized by the yeast two-hybrid
system introduced by Fields and coworkers. In recent years, similar genetic assays have …
system introduced by Fields and coworkers. In recent years, similar genetic assays have …
Screening Peptide/Protein Libraries Fused to the λ Repressor DNA-Binding Domain in E. coli Cells
L Mariño-Ramírez, L Campbell, JC Hu - E. coli Gene Expression Protocols, 2003 - Springer
The use of λ repressor fusions to study protein-protein interactions in E. coli was first
described by Hu and others (1). Since then, the repressor system has been employed by …
described by Hu and others (1). Since then, the repressor system has been employed by …
A genetic screen to identify sequences that mediate protein oligomerization in Escherichia coli
R Jappelli, S Brenner - Biochemical and Biophysical Research …, 1999 - Elsevier
Many proteins assemble as oligomeric complexes and in several cases a distinct domain
mediates the interaction between the subunits. The identification of new oligomerization …
mediates the interaction between the subunits. The identification of new oligomerization …
Ultrasound-mediated Gene Transfer to Enhance Bioremediation of Contaminated Water
DG Boardman - 2014 - etheses.whiterose.ac.uk
A novel technique for in situ bioremediation is vital to enable the world to meet the need to
treat contaminated land; ultrasound gene transfer has that potential. Ultrasound gene …
treat contaminated land; ultrasound gene transfer has that potential. Ultrasound gene …
Screening Peptide/Protein Libraries Fused to the Repressor DNA-Binding Domain in E. coli Cells
L Mariño-Ramírez, L Campbell, JC Hu - E. coli Gene Expression Protocols - Springer
The use of repressor fusions to study protein-protein interactions in E. coli was first
described by Hu and others (1). Since then, the repressor system has been employed by …
described by Hu and others (1). Since then, the repressor system has been employed by …