Recent advances on the multiplex molecular detection of plant viruses and viroids

V Pallás, JA Sánchez-Navarro, D James - Frontiers in microbiology, 2018 - frontiersin.org
Plant viruses are still one of the main contributors to economic losses in agriculture. It has
been estimated that plant viruses can cause as much as 50 billion euros loss worldwide, per …

Ilarviruses of Prunus spp.: A Continued Concern for Fruit Trees

V Pallas, F Aparicio, MC Herranz, K Amari… - …, 2012 - Am Phytopath Society
Prunus spp. are affected by a large number of viruses, causing significant economic losses
through either direct or indirect damage, which results in reduced yield and fruit quality …

Simultaneous detection of nine grapevine viruses by multiplex reverse transcription-polymerase chain reaction with coamplification of a plant RNA as internal control

G Gambino, I Gribaudo - Phytopathology, 2006 - Am Phytopath Society
ABSTRACT A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was
developed for simultaneous detection of nine grapevine viruses: Arabis mosaic virus …

Control of pome and stone fruit virus diseases

M Barba, V Ilardi, G Pasquini - Advances in virus research, 2015 - Elsevier
Many different systemic pathogens, including viruses, affect pome and stone fruits causing
diseases with adverse effects in orchards worldwide. The significance of diseases caused …

[图书][B] Plant virus and viroid diseases in the tropics

KS Sastry, TA Zitter - 2014 - Springer
Many of the world's most important food crops are grown in the tropics and major crops like
rice, maize, wheat, sorghum, barley, tomato, chillies, okra, peas, peanut, sunflower …

Microarrays for rapid identification of plant viruses

N Boonham, J Tomlinson, R Mumford - Annu. Rev. Phytopathol., 2007 - annualreviews.org
Many factors affect the development and application of diagnostic techniques. Plant viruses
are an inherently diverse group that, unlike cellular pathogens, possess no nucleotide …

Novel approaches to mitigate primer interaction and eliminate inhibitors in multiplex PCR, demonstrated using an assay for detection of three strawberry viruses

T Wei, G Lu, G Clover - Journal of Virological Methods, 2008 - Elsevier
Multiplex PCR is an important technique for detecting a variety of pathogens simultaneously
in a single assay. Previous research has focused on optimising the factors affecting reliable …

Assessment of virus eradication methods from infected in vitro-grown apricot cultures

A Zare Khafri, R Zarghami, M Naderpour… - Plant Cell, Tissue and …, 2024 - Springer
In vitro culture of apricot cultivars 'Qaysi','Shams', and 'Ordubad', infected with Apple chlorotic
leaf spot virus (ACLSV), Apple mosaic virus (ApMV), and Tobacco ring spot virus (TRSV) …

Targeted genome sequencing (TG-Seq) approaches to detect plant viruses

S Maina, L Zheng, BC Rodoni - Viruses, 2021 - mdpi.com
Globally, high-throughput sequencing (HTS) has been used for virus detection in germplasm
certification programs. However, sequencing costs have impeded its implementation as a …

Recovery of virus-free Almond (Prunus dulcis) cultivars by somatic embryogenesis from meristem undergone thermotherapy

M Ebrahimi, AA Habashi, M Emadpour, N Kazemi - Scientific Reports, 2022 - nature.com
One of the world's main horticulture problems is the contamination of fruit trees with a variety
of plant diseases, especially viral and pseudo-viral diseases. Due to the non-sexual …