Prime editing installs precise edits into the genome with minimal unwanted byproducts, but
low and variable editing efficiencies have complicated application of the approach to high …

Background High-efficiency prime editing (PE) is desirable for precise genome
manipulation. The activity of mammalian PE systems can be largely improved by inhibiting …

Personalized cancer therapeutics bring directed treatment options to patients based on their
tumor's genetic signature. Unfortunately, tumor genomes are remarkably adaptable, and …

The prime editing 2 (PE2) system comprises a nickase Cas9 fused to a reverse transcriptase
utilizing a prime editing guide RNA (pegRNA) to introduce desired mutations at target …

Abstract Type 2 diabetes (T2D) affects~ 500 million individuals worldwide and is projected to
rise 46% by 2045. Patient care is complicated by T2D's heterogeneous nature and …

The medical community is on the cusp of revolutionary curative treatments through
therapeutic genome editing. However, technical limitations, such as unintentional and …

Engineering of CRISPR-Cas systems has produced a suite of genome editing tools
including programmable nucleases, base editors and prime editors. During my Ph. D., I …