Human T cell development, localization, and function throughout life
BV Kumar, TJ Connors, DL Farber - Immunity, 2018 - cell.com
Throughout life, T cells coordinate multiple aspects of adaptive immunity, including
responses to pathogens, allergens, and tumors. In mouse models, the role of T cells is …
responses to pathogens, allergens, and tumors. In mouse models, the role of T cells is …
Location, location, location: Tissue resident memory T cells in mice and humans
The discovery of T cells resident in diverse tissues has altered our understanding of
adaptive immunity to encompass site-specific responses mediated by tissue-adapted …
adaptive immunity to encompass site-specific responses mediated by tissue-adapted …
Cross-tissue immune cell analysis reveals tissue-specific features in humans
C Domínguez Conde, C Xu, LB Jarvis, DB Rainbow… - Science, 2022 - science.org
Despite their crucial role in health and disease, our knowledge of immune cells within
human tissues remains limited. We surveyed the immune compartment of 16 tissues from 12 …
human tissues remains limited. We surveyed the immune compartment of 16 tissues from 12 …
Initialization is critical for preserving global data structure in both t-SNE and UMAP
D Kobak, GC Linderman - Nature biotechnology, 2021 - nature.com
Our aim here was not to argue which algorithm, t-SNE or UMAP, is more suitable for single-
cell studies. Once informative initialization is used, the two algorithms appear to preserve the …
cell studies. Once informative initialization is used, the two algorithms appear to preserve the …
Dimensionality reduction for visualizing single-cell data using UMAP
Advances in single-cell technologies have enabled high-resolution dissection of tissue
composition. Several tools for dimensionality reduction are available to analyze the large …
composition. Several tools for dimensionality reduction are available to analyze the large …
Ulcerative colitis is characterized by a plasmablast-skewed humoral response associated with disease activity
B cells, which are critical for intestinal homeostasis, remain understudied in ulcerative colitis
(UC). In this study, we recruited three cohorts of patients with UC (primary cohort, n= 145; …
(UC). In this study, we recruited three cohorts of patients with UC (primary cohort, n= 145; …
Defining inflammatory cell states in rheumatoid arthritis joint synovial tissues by integrating single-cell transcriptomics and mass cytometry
To define the cell populations that drive joint inflammation in rheumatoid arthritis (RA), we
applied single-cell RNA sequencing (scRNA-seq), mass cytometry, bulk RNA sequencing …
applied single-cell RNA sequencing (scRNA-seq), mass cytometry, bulk RNA sequencing …
[HTML][HTML] Single-cell analysis of Crohn's disease lesions identifies a pathogenic cellular module associated with resistance to anti-TNF therapy
Clinical benefits of cytokine blockade in ileal Crohn's disease (iCD) are limited to a subset of
patients. Here, we applied single-cell technologies to iCD lesions to address whether …
patients. Here, we applied single-cell technologies to iCD lesions to address whether …
Guidelines for the use of flow cytometry and cell sorting in immunological studies
A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …
immunology and flow cytometry community. They provide the theory and key practical …
Human tissue-resident memory T cells are defined by core transcriptional and functional signatures in lymphoid and mucosal sites
Tissue-resident memory T cells (TRMs) in mice mediate optimal protective immunity to
infection and vaccination, while in humans, the existence and properties of TRMs remain …
infection and vaccination, while in humans, the existence and properties of TRMs remain …