Bi-directional cell-pericellular matrix interactions direct stem cell fate

SA Ferreira, MS Motwani, PA Faull, AJ Seymour… - Nature …, 2018 - nature.com
Modifiable hydrogels have revealed tremendous insight into how physical characteristics of
cells' 3D environment drive stem cell lineage specification. However, in native tissues, cells …

Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration

P Sawadkar, N Mandakhbayar… - Journal of tissue …, 2021 - journals.sagepub.com
Current gold standard to treat soft tissue injuries caused by trauma and pathological
condition are autografts and off the shelf fillers, but they have inherent weaknesses like …

A synergistic relationship between polycaprolactone and natural polymers enhances the physical properties and biological activity of scaffolds

P Sawadkar, J Mohanakrishnan… - … applied materials & …, 2020 - ACS Publications
Biomaterials for tissue engineering include natural and synthetic polymers, but their clinical
application is still limited due to various disadvantages associated with the use of these …

Influence of periodontal ligament stem cell-derived conditioned medium on osteoblasts

S Novello, S Tricot-Doleux, A Novella, P Pellen-Mussi… - Pharmaceutics, 2022 - mdpi.com
Mesenchymal stem cells (MSC) are involved in the regeneration of various missing or
compromised periodontal tissues, including bone. MSC-derived conditioned medium (CM) …

HWJMSC-derived extracellular vesicles ameliorate IL-1β-induced chondrocyte injury through regulation of the BMP2/RUNX2 axis via up-regulation TFRC

Z Chen, W Ding, P Duan, X Lv, Y Feng, Z Yin, Z Luo… - Cellular …, 2023 - Elsevier
Articular osteochondral injury is a common and frequently occurring disease in orthopedics
that is caused by aging, disease, and trauma. The cytokine interleukin-1β (IL-1β) is a crucial …

RT-qPCR analyses on the osteogenic differentiation from human iPS cells: an investigation of reference genes

K Okamura, Y Inagaki, TK Matsui, M Matsubayashi… - Scientific Reports, 2020 - nature.com
Reverse transcription quantitative PCR (RT-qPCR) is used to quantify gene expression and
require standardization with reference genes. We sought to identify the reference genes best …

Physicochemical properties, cytocompatibility, and biocompatibility of a bioactive glass based retrograde filling material

K Murata, A Washio, T Morotomi, T Rojasawasthien… - Nanomaterials, 2021 - mdpi.com
The ideal retrograde filling material that is easy to handle, has good physicochemical
properties, and is biocompatible has not yet been developed. The current study reports the …

Cell culture media notably influence properties of human mesenchymal stroma/stem-like cells from different tissues

A Winkel, Y Jaimes, C Melzer, P Dillschneider… - Cytotherapy, 2020 - Elsevier
Background aims Mesenchymal stroma/stem-like cells (MSCs) are a popular cell source and
hold huge therapeutic promise for a broad range of possible clinical applications. However …

Identification of reference genes for qPCR analysis during hASC long culture maintenance

S Palombella, C Pirrone, M Cherubino, L Valdatta… - PLoS …, 2017 - journals.plos.org
Up to now quantitative PCR based assay is the most common method for characterizing or
confirming gene expression patterns and comparing mRNA levels in different sample …

Validation of housekeeping genes as reference for reverse‐transcription‐qPCR analysis in busulfan‐injured microvascular endothelial cells

W Ju, AO Smith, T Sun, P Zhao, Y Jiang… - BioMed Research …, 2018 - Wiley Online Library
Endothelial cells (ECs) could express some important cytokines and signal molecules which
play a key role in normal hematopoiesis and repopulation. Busulfan‐induced vascular …