Recent developments in bioprocessing of recombinant proteins: expression hosts and process development

NK Tripathi, A Shrivastava - Frontiers in Bioengineering and …, 2019 - frontiersin.org
Infectious diseases, along with cancers, are among the main causes of death among
humans worldwide. The production of therapeutic proteins for treating diseases at large …

Economic analysis of batch and continuous biopharmaceutical antibody production: A review

O Yang, M Qadan, M Ierapetritou - Journal of pharmaceutical innovation, 2020 - Springer
Purpose There is a growing interest in continuous biopharmaceutical processing due to the
advantages of small footprint, increased productivity, consistent product quality, high …

The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody purification process

CEM Hogwood, AS Tait… - Biotechnology and …, 2013 - Wiley Online Library
Recombinant protein products such as monoclonal antibodies (mAbs) for use in the clinic
must be clear of host cell impurities such as host cell protein (HCP), DNA/RNA, and high …

Measurement and control of host cell proteins (HCPs) in CHO cell bioprocesses

CEM Hogwood, DG Bracewell, CM Smales - Current opinion in …, 2014 - Elsevier
Highlights•Cell viability and time of harvest impacts greatly on the subsequent HCP
profile.•Primary and secondary clarification decisions impact on the HCP profile.•HCPs in …

Host cell protein removal from biopharmaceutical preparations: Towards the implementation of quality by design

CH Goey, S Alhuthali, C Kontoravdi - Biotechnology advances, 2018 - Elsevier
Downstream processing of protein products of mammalian cell culture currently accounts for
the largest fraction of the total production cost. A major challenge is the removal of host cell …

Dynamic simulation, optimisation and economic analysis of fed-batch vs. perfusion bioreactors for advanced mAb manufacturing

W Jones, DI Gerogiorgis - Computers & Chemical Engineering, 2022 - Elsevier
The variation of antigen binding sites on monoclonal antibodies (mAbs) enables
manufacturing of the latter for targeted treatment of a variety of ailments, including cancer …

Host cell protein dynamics in recombinant CHO cells: impacts from harvest to purification and beyond

CEM Hogwood, DG Bracewell, CM Smales - Bioengineered, 2013 - Taylor & Francis
During the production of recombinant protein products, such as monoclonal antibodies,
manufacturers must demonstrate clearance of host cell impurities and contaminants to …

The application of hollow fiber cartridge in biomedicine

Y Hou, K Mi, L Sun, K Zhou, L Wang, L Zhang, Z Liu… - Pharmaceutics, 2022 - mdpi.com
The hollow fiber cartridge has the advantages of good semi-permeability, high surface area
to volume ratio, convenient operation, and so on. Its application in chemical analysis, drug in …

FYWHCLDE-based affinity chromatography of IgG: Effect of ligand density and purifications of human IgG and monoclonal antibody

WW Zhao, QH Shi, Y Sun - Journal of Chromatography A, 2014 - Elsevier
This work reports the development of an octapeptide-based affinity adsorbent for the
purification of human IgG (hIgG) and monoclonal antibody (mAb). The octapeptide was …

One-step integrated clarification and purification of a monoclonal antibody using Protein A Mag Sepharose beads and a cGMP-compliant high-gradient magnetic …

M Ebeler, O Lind, N Norrman, R Palmgren… - New biotechnology, 2018 - Elsevier
Monoclonal antibodies are a dominant component of today's biopharmaceutical market and
are typically purified by classical platform processes. However, high costs and rising …