Production of highly soluble and immuno-reactive recombinant flagellin protein of Clostridium chauvoei

A Prajapati, RA Hemanth, MR Namrutha, S Bindu… - Anaerobe, 2024 - Elsevier
Objective Flagellin protein, an integral component of flagella, provides motility to several
bacterial species and also acts as a candidate antigen in diagnostics and subunit vaccines …

A colloidal gold immunochromatographic strip assay for the rapid detection of Shigella in milk and meat products

B Ang, X Xu, L Liu, L Xu, H Kuang, C Xu - New Journal of Chemistry, 2022 - pubs.rsc.org
Shigella is the most common foodborne pathogen and can cause bacillary dysentery.
Therefore, it is necessary to detect Shigella rapidly and accurately, but it is difficult for …

A multi-species indirect ELISA for detection group-specific antibodies against VP7 protein of bluetongue virus

K Chand, SK Biswas, MA Ramakrishnan - Small Ruminant Research, 2019 - Elsevier
In the present study, a multispecies indirect ELISA for the detection of serogroup-specific
antibodies against the VP7 protein of bluetongue virus (BTV) has been developed. The …

Development of ELISA for the detection of antibodies against VP2 protein of bluetongue virus serotype-1

CS Mahapatra, P Sharma, SK Biswas… - Journal of Immunological …, 2022 - Elsevier
Serotype-specific diagnosis of bluetongue virus (BTV) is necessary for sero-surveillance and
taking effective control measures. The VP2 is the major serotype determining protein and …

Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep

NN Mohanty, D Hemadri… - Journal of …, 2021 - Elsevier
Bluetongue is an insect borne (Culicoides) viral disease of small ruminants. The virus
blankets the globe with a wide serotypic variation, numbered from 1 to 28. In India 21 …

A critical challenge in the development of antibody: Selecting the appropriate fragment of the target protein as an antigen based on various epitopes or similar …

M Razzaqi, MJ Rasaee, M Paknejad - Molecular immunology, 2019 - Elsevier
The main challenge in the development of antibody is to select the appropriate antigen
particularly when a truncated protein is used for immunization or as vaccine antigen. In …

Co-administration of recombinant major envelope proteins (rA27L and rH3L) of buffalopox virus provides enhanced immunogenicity and protective efficacy in animal …

A Kumar, R Yogisharadhya, G Venkatesan… - Antiviral Research, 2017 - Elsevier
Buffalopox virus (BPXV) and other vaccinia-like viruses (VLVs) are causing an emerging/re-
emerging zoonosis affecting buffaloes, cattle and humans in India and other countries. A27L …

An efficient production of hybrid recombinant protein comprising non-structural proteins (NS 1 & NS 3) of bluetongue virus in prokaryotic expression system

NN Mohanty, SB Shivachandra, SK Biswas… - Protein Expression and …, 2019 - Elsevier
Strategic design and suitable purification techniques are of paramount importance in the
production of recombinant proteins, if intended for use in a diagnostic assay. However, there …

[PDF][PDF] Immuno-reactivity of recombinant non-structural protein 3 N-terminus (rNS3Nt) in indirect-ELISA for detection of bluetongue viral antibodies in serum samples

N Chacko, SK Biswas, NN Mohanty… - The Indian Journal of …, 2017 - epubs.icar.org.in
Bluetongue, an arthropod borne non-contagious disease of ruminants especially sheep, is
caused by bluetongue virus (BTV). Detection of BTV antibodies in susceptible hosts is …

Comparative immuno-reactivity of recombinant non-structural protein 2 fragments (N-and C-terminus) to detect bluetongue viral antibodies in small ruminant serum …

N Chacko, SK Biswas, NN Mohanty, K Chand… - Small Ruminant …, 2019 - Elsevier
Bluetongue viral antibody detection assays are important for routine disease diagnosis and
sero-surveillance in susceptible host's especially small ruminants. In the present study, a …