Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology
Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …
of isolated macromolecules to near-atomic resolution. However, these data do not provide …
[HTML][HTML] Cell death by cornification
L Eckhart, S Lippens, E Tschachler… - Biochimica et Biophysica …, 2013 - Elsevier
Epidermal keratinocytes undergo a unique form of terminal differentiation and programmed
cell death known as cornification. Cornification leads to the formation of the outermost skin …
cell death known as cornification. Cornification leads to the formation of the outermost skin …
Serial Lift-Out: sampling the molecular anatomy of whole organisms
Cryo-focused ion beam milling of frozen-hydrated cells and subsequent cryo-electron
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …
Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells
INTRODUCTION Our textbook understanding of the nanoscale organization of the cell and
its relationship to the thousands of proteins that drive cellular metabolism comes largely from …
its relationship to the thousands of proteins that drive cellular metabolism comes largely from …
Quantification of gallium cryo-FIB milling damage in biological lamellae
BA Lucas, N Grigorieff - Proceedings of the National …, 2023 - National Acad Sciences
Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological
processes in their native, cellular environment at atomic resolution. However, few cells are …
processes in their native, cellular environment at atomic resolution. However, few cells are …
[HTML][HTML] An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology
Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …
A technical introduction to transmission electron microscopy for soft‐matter: Imaging, possibilities, choices, and technical developments
With a significant role in material sciences, physics,(soft matter) chemistry, and biology, the
transmission electron microscope is one of the most widely applied structural analysis tool to …
transmission electron microscope is one of the most widely applied structural analysis tool to …
Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography
FR Wagner, R Watanabe, R Schampers, D Singh… - Nature protocols, 2020 - nature.com
Recent advances have made cryogenic (cryo) electron microscopy a key technique to
achieve near-atomic-resolution structures of biochemically isolated macromolecular …
achieve near-atomic-resolution structures of biochemically isolated macromolecular …
Outcome of the first electron microscopy validation task force meeting
R Henderson, A Sali, ML Baker, B Carragher… - Structure, 2012 - cell.com
This Meeting Review describes the proceedings and conclusions from the inaugural
meeting of the Electron Microscopy Validation Task Force organized by the Unified Data …
meeting of the Electron Microscopy Validation Task Force organized by the Unified Data …
Cryo-electron tomography: the challenge of doing structural biology in situ
Electron microscopy played a key role in establishing cell biology as a discipline, by
producing fundamental insights into cellular organization and ultrastructure. Many seminal …
producing fundamental insights into cellular organization and ultrastructure. Many seminal …