Vibrational spectroscopy provides a direct route to the physicochemical characterization of
molecules. While both IR and Raman spectroscopy have been used for decades to provide …

Background Most biological processes involve water, and the interactions of biomolecules
with water affect their structure, function and dynamics. Scope of review This review …

Water is arguably the most common and yet least understood material on Earth. Indeed, the
biophysical behavior of water in crowded intracellular milieu is a long-debated issue …

The enzyme Candida Antarctica lipase B (CALB) serves here as a model for understanding
connections among hydration layer dynamics, solvation shell structure, and protein surface …

Proteins are complex, heterogeneous macromolecules that exist as ensembles of
interconverting states on a complex energy landscape. A complete, molecular-level …

Fried et al.(Reports, 19 December 2014, p. 1510) demonstrate electric field–dependent
acceleration of biological catalysis using ketosteroid isomerase as a prototypic example …

The interplay between the intracellular gate and the selectivity filter underlies the structural
basis for gating in potassium ion channels. Using a combination of protein semisynthesis …

Water molecules in the immediate vicinity of biomacromolecules, including proteins,
constitute a hydration layer characterized by physicochemical properties different from those …

The solvation layer surrounding a protein is clearly an intrinsic part of protein structure–
dynamics–function, and our understanding of how the hydration dynamics influences protein …

Mycobacterium tuberculosis pyrazinamidase (PZAse) is a key enzyme to activate the pro-
drug pyrazinamide (PZA). PZAse is a metalloenzyme that coordinates in vitro different …