Nonintegrating gene therapy vectors
T Athanasopoulos, MM Munye… - Hematology …, 2017 - hemonc.theclinics.com
The concept of gene therapy arose during the 1960s and early 1970s. Rogers and Pfuderer
1 carried out the first genetic modification of a virus (Tobacco mosaic virus [TMV]), and …
1 carried out the first genetic modification of a virus (Tobacco mosaic virus [TMV]), and …
Yeast artificial chromosomes: tools for mapping and analysis of complex genomes
D Schlessinger - Trends in Genetics, 1990 - cell.com
Contigs for chromosome V of C. elegans. Contigs are represented by the solid bars above,
with vertical lines showing the positions of genetic markers. YAC distribution is represented …
with vertical lines showing the positions of genetic markers. YAC distribution is represented …
Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents.
HM Albertsen, H Abderrahim… - Proceedings of the …, 1990 - National Acad Sciences
Prior to constructing a library of yeast artificial chromosomes (YACs) containing very large
human DNA fragments, we performed a series of preliminary experiments aimed at …
human DNA fragments, we performed a series of preliminary experiments aimed at …
Isolation of single-copy human genes from a library of yeast artificial chromosome clones
A recently developed cloning system based on the propagation of large DNA molecules as
linear, artificial chromosomes in the yeast Saccharomyces cerevisiae provides a potential …
linear, artificial chromosomes in the yeast Saccharomyces cerevisiae provides a potential …
Detection and characterization of chimeric yeast artificial-chromosome clones
ED Green, HC Riethman, JE Dutchik, MV Olson - Genomics, 1991 - Elsevier
Methods for the construction of yeast artificial-chromosome (YAC) clones have been
designed to isolate single, large (100–1000 kb) segments of chromosomal DNA. It is …
designed to isolate single, large (100–1000 kb) segments of chromosomal DNA. It is …
Cl- Channels in CF: Lack of Activation by Protein Kinase C and cAMP-Dependent Protein Kinase
Secretory chloride channels can be activated by adenosine 3′, 5′-monophosphate
(cAMP)-dependent protein kinase in normal airway epithelial cells but not in cells from …
(cAMP)-dependent protein kinase in normal airway epithelial cells but not in cells from …
Amplification of large artificial chromosomes.
DR Smith, AP Smyth, DT Moir - Proceedings of the National …, 1990 - National Acad Sciences
Yeast artificial chromosome cloning is an attractive technology for genomic mapping studies
because very large DNA segments can be readily propagated. However, detailed analyses …
because very large DNA segments can be readily propagated. However, detailed analyses …
Deletions in Xq28 in two boys with myotubular myopathy and abnormal genital development define a new contiguous gene syndrome in a 430 kb region
LJ Hu, J Laporte, W Kress, P Kioschis… - Human molecular …, 1996 - academic.oup.com
We have recently described a female patient with myotubular myopathy (MTM1) and an
interstitial deletion at Xq28. Characterisation of the deletion allowed us to position the MTM1 …
interstitial deletion at Xq28. Characterisation of the deletion allowed us to position the MTM1 …
[17] Preparation of clone libraries in yeast artificial-chromosome vectors
DT Burke, MV Olson - Methods in enzymology, 1991 - Elsevier
Publisher Summary This chapter describes the use of pYAC4 for cloning large fragments of
human DNA prepared by partial digestion with EcoRI. Straightforward modifications of these …
human DNA prepared by partial digestion with EcoRI. Straightforward modifications of these …
Physical mapping of the Myxococcus xanthus genome by random cloning in yeast artificial chromosomes.
A KuSPA, D Vollrath, Y Cheng… - Proceedings of the …, 1989 - National Acad Sciences
Random segments of Myxococcus xanthus DNA were cloned in yeast artificial
chromosomes (YACs) to construct a physical map of the genome. EcoRI restriction maps of …
chromosomes (YACs) to construct a physical map of the genome. EcoRI restriction maps of …