Viral delivery of dsRNA for control of insect agricultural pests and vectors of human disease: prospects and challenges

A Kolliopoulou, CNT Taning, G Smagghe… - Frontiers in …, 2017 - frontiersin.org
RNAi is applied as a new and safe method for pest control in agriculture but efficiency and
specificity of delivery of dsRNA trigger remains a critical issue. Various agents have been …

Transcription factors, CncC and Maf, regulate expression of CYP6BQ genes responsible for deltamethrin resistance in Tribolium castaneum

M Kalsi, SR Palli - Insect Biochemistry and Molecular Biology, 2015 - Elsevier
Insecticide resistance is a global problem that presents an ongoing challenge to control
insects that destroy crops, trees and transmit diseases. Dramatic progress has been made …

Mechanisms of cell entry by dsRNA viruses: Insights for efficient delivery of dsRNA and tools for improved RNAi-based pest control

L Swevers, D Kontogiannatos, A Kolliopoulou… - Frontiers in …, 2021 - frontiersin.org
While RNAi is often heralded as a promising new strategy for insect pest control, a major
obstacle that still remains is the efficient delivery of dsRNA molecules within the cells of the …

Persistent RNA virus infection of lepidopteran cell lines: interactions with the RNAi machinery

L Swevers, K Ioannidis, M Kolovou, A Zografidis… - Journal of insect …, 2016 - Elsevier
RNAi is broadly used as a technique for specific gene silencing in insects but few studies
have investigated the factors that can affect its efficiency. Viral infections have the potential …

Analysis of luciferase dsRNA production during baculovirus infection of Hi5 cells: RNA hairpins expressed by very late promoters do not trigger gene silencing

A Kolliopoulou, D Kontogiannatos… - Frontiers in Insect …, 2022 - frontiersin.org
The baculovirus expression vector system (BEVS) has become an important platform for the
expression of recombinant proteins and is especially useful for the production of large …

Short-term persistence precedes pathogenic infection: Infection kinetics of cricket paralysis virus in silkworm-derived Bm5 cells

L Wang, K Cappelle, D Santos, JV Broeck… - Journal of insect …, 2019 - Elsevier
Next generation sequencing has revealed the widespread occurrence of persistent virus
infections in insects but little is known regarding to what extent persistent infections can …

Antiviral defense against Cypovirus 1 (Reoviridae) infection in the silkworm, Bombyx mori

L Swevers, M Feng, F Ren, J Sun - Archives of insect …, 2020 - Wiley Online Library
Recent years have shown a large increase in studies of infection of the silkworm (Bombyx
mori) with Cypovirus 1 (previously designated as B. mori cytoplasmic polyhedrosis virus) …

Stimulation of IRES‐Dependent Translation by Rocaglamide A Increases the Replication and Virulence of Cricket Paralysis Virus in Lepidopteran Insect Cells

D Santos, VM Christopoulou… - Archives of Insect …, 2025 - Wiley Online Library
The discovery that infections of viruses are pervasive among insects has considerable
potential for future applications, such as new strategies for pest control through the …

昆虫RNA 干扰中双链RNA 的转运方式

刘吉升, 朱文辉, 廖文丽, 卜晓玲, 江婉仪 - 昆虫学报, 2016 - insect.org.cn
昆虫RnA 干扰主要指外源双链RnA 诱发的, 通过阻碍特定基因的翻译或转录,
引起内源目标信使RnA 沉默的机制. 由于RnA 干扰的特异性, RnA 干扰技术主要应用于昆虫 …

Transcriptional response of immune-related genes after endogenous expression of VP1 and exogenous exposure to VP1-based VLPs and CPV virions in …

Y Zhao, A Kolliopoulou, F Ren, Q Lu… - Molecular Genetics and …, 2019 - Springer
In insects, RNAi is considered the major antiviral immune defense pathway. DsRNAs
produced during viral infection are processed by Dicer enzymes into small RNAs that …