Immobilization of β-galactosidase on concanavalin A modified silica-coated titanium dioxide nanocomposite and its interaction with monovalent and divalent cations

A Shafi, Q Husain - Materials Today Communications, 2022 - Elsevier
In this report, we describe a bioaffinity based immobilization protocol of β-galactosidase on
silica-coated titanium dioxide nanocomposite modified using concanavalin A. The prepared …

[HTML][HTML] The mineral profile affects the coagulation pattern and cheese-making efficiency of bovine milk

G Stocco, A Summer, C Cipolat-Gotet… - Journal of Dairy …, 2021 - Elsevier
Natural variations in milk minerals, their relationships, and their associations with the
coagulation process and cheese-making traits present an opportunity for the differentiation …

Engineering the performance of artificial inclusion bodies built of catalytic β-galactosidase

JM Sanchez, H Lopez-Laguna, N Serna… - ACS Sustainable …, 2021 - ACS Publications
One of the most critical bottlenecks in the application of industrial enzymes is the
preservation of protein stability throughout the catalytic reaction, which often requires protein …

Purification and characterization of β-galactosidase from newly isolated Aspergillus terreus (KUBCF1306) and evaluating its efficacy on breast cancer cell line (MCF-7 …

B Vidya, M Palaniswamy, J Angayarkanni… - Bioorganic …, 2020 - Elsevier
Abstract β-galactosidases (EC 3.2. 1.23) are able to catalyze two different types of reactions,
namely hydrolysis and transgalactosylation. It is a lysosomal exoglycosidase involved in the …

Parallel Dielectrophoretic Capture, Isolation, and Electrical Lysis of Individual Breast Cancer Cells to Assess Variability in Enzymatic Activity

JT Banovetz, S Manimaran, BT Schelske… - Analytical …, 2023 - ACS Publications
Tumor cell heterogeneity drives disease progression and response to therapy, and
therefore, there is a need for single-cell analysis methods. In this paper, we present an …

Stabilization of β-D-galactosidase in solution containing chitosan-based membrane: Central composite rotatable design

LF da Cruz, AG Polizeli, H Enzweiler… - International Journal of …, 2024 - Elsevier
Abstract β-D-galactosidase is a hydrolase enzyme capable of hydrolyzing lactose in milk-
based foods. Its free form can be inactivated in solution during the production of low-dosage …

A Novel β-Galactosidase from Kluyvera intermedia and its Potential for Hydrolyzing Lactose in Milk

Y Liu, S Lutz-Wahl, L Kettner, L Fischer - Food Biotechnology, 2024 - Taylor & Francis
ABSTRACT A new β-galactosidase (EC 3.2. 1.23) discovered in Kluyvera intermedia (β-gal-
Kli) was recombinantly produced in Escherichia coli BL21 (DE3). The bioreactor cultivation …

[HTML][HTML] Asymmetrical flow field-flow fractionation to probe the dynamic association equilibria of β-D-galactosidase

IK Ventouri, A Astefanei, ER Kaal, R Haselberg… - … of Chromatography A, 2021 - Elsevier
Protein dynamics play a significant role in many aspects of enzyme activity. Monitoring of
structural changes and aggregation of biotechnological enzymes under native conditions is …

基于最适营养解析的瑞士乳杆菌H11 高密度培养基优化.

聂新, 宗晓婕, 武弘理, 刘凯龙… - Journal of Chinese …, 2024 - search.ebscohost.com
摘要目的: 解析瑞士乳杆菌H11 的最适营养需求, 探究其成本低, 活菌数高的培养基配方, 方法:
以活菌数, 菌体密度, 最大比生长速率为指标, 通过单因素实验, Box) Behnken 响应面试验设计 …

Production and partial purification of β-galactosidase enzyme from probiotic Bacillus subtilis SK09

GC Percival, M Chamundeeswari, RF Lovlyna… - 2019 - nopr.niscpr.res.in
In the present study, lactose hydrolyzing enzyme β-galactosidase was produced from a
novel probiotic Bacillus subtilis SK09 and it was compared with commercially obtained, B …