The promise and the challenges of cryo‐electron tomography
M Turk, W Baumeister - FEBS letters, 2020 - Wiley Online Library
Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …
manner in which the cellular molecular components are fractionated and purified before …
Better, faster, cheaper: recent advances in cryo–electron microscopy
Cryo–electron microscopy (cryo-EM) continues its remarkable growth as a method for
visualizing biological objects, which has been driven by advances across the entire pipeline …
visualizing biological objects, which has been driven by advances across the entire pipeline …
High-throughput cryo-ET structural pattern mining by unsupervised deep iterative subtomogram clustering
Cryoelectron tomography directly visualizes heterogeneous macromolecular structures in
their native and complex cellular environments. However, existing computer-assisted …
their native and complex cellular environments. However, existing computer-assisted …
Quality assessment of the neural algorithms on the example of EIT-UST hybrid tomography
The paper presents the results of research on the hybrid industrial tomograph electrical
impedance tomography (EIT) and ultrasonic tomography (UST)(EIT-UST), operating on the …
impedance tomography (EIT) and ultrasonic tomography (UST)(EIT-UST), operating on the …
Unravelling molecular complexity in structural cell biology
S Pfeffer, J Mahamid - Current opinion in structural biology, 2018 - Elsevier
Highlights•Cryo-ET and computational subtomogram averaging have attained resolutions
suitable for atomic model building.•Cellular cryo-ET can visualize macromolecules at near-to …
suitable for atomic model building.•Cellular cryo-ET can visualize macromolecules at near-to …
Big data in cryoEM: automated collection, processing and accessibility of EM data
Highlights•Cryogenic electron microscopy has been evolving towards a big data field.•Movie-
mode collection and automation has increased throughput.•Improved processing pipelines …
mode collection and automation has increased throughput.•Improved processing pipelines …
Cryomicroscopy in situ: what is the smallest molecule that can be directly identified without labels in a cell?
Electron cryomicroscopy (cryoEM) has made great strides in the last decade, such that the
atomic structure of most biological macromolecules can, at least in principle, be determined …
atomic structure of most biological macromolecules can, at least in principle, be determined …
Weakly supervised 3d semantic segmentation using cross-image consensus and inter-voxel affinity relations
We propose a novel weakly supervised approach for 3D semantic segmentation on
volumetric images. Unlike most existing methods that require voxel-wise densely labeled …
volumetric images. Unlike most existing methods that require voxel-wise densely labeled …
Improved deep learning-based macromolecules structure classification from electron cryo-tomograms
Cellular processes are governed by macromolecular complexes inside the cell. Study of the
native structures of macromolecular complexes has been extremely difficult due to lack of …
native structures of macromolecular complexes has been extremely difficult due to lack of …
Neural image reconstruction using a heuristic validation mechanism
D Połap, G Srivastava - Neural Computing and Applications, 2021 - Springer
Image reconstruction is a mathematical process, where the image is compressed into a
small representation and derived from this form. The general use of the reconstruction …
small representation and derived from this form. The general use of the reconstruction …