Genetically encoded multimeric tags for subcellular protein localization in cryo-EM
Cryo-electron tomography (cryo-ET) allows for label-free high-resolution imaging of
macromolecular assemblies in their native cellular context. However, the localization of …
macromolecular assemblies in their native cellular context. However, the localization of …
RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin
The small GTPase ARL8 associates with endolysosomes, leading to the recruitment of
several effectors that couple endolysosomes to kinesins for anterograde transport along …
several effectors that couple endolysosomes to kinesins for anterograde transport along …
[HTML][HTML] From observing to controlling: Inducible control of organelle dynamics and interactions
The dynamics and interactions of cellular organelles underlie many aspects of cellular
functioning. Until recently, assessment of organelle dynamics has been primarily …
functioning. Until recently, assessment of organelle dynamics has been primarily …
The evolution of the axonal transport toolkit
Neurons are highly polarized cells that critically depend on long‐range, bidirectional
transport between the cell body and synapse for their function. This continual and highly …
transport between the cell body and synapse for their function. This continual and highly …
[HTML][HTML] Synaptic vesicle precursors and lysosomes are transported by different mechanisms in the axon of mammalian neurons
BORC is a multisubunit complex previously shown to promote coupling of mammalian
lysosomes and C. elegans synaptic vesicle (SV) precursors (SVPs) to kinesins for …
lysosomes and C. elegans synaptic vesicle (SV) precursors (SVPs) to kinesins for …
[HTML][HTML] An optimized toolbox for the optogenetic control of intracellular transport
W Nijenhuis, MMP van Grinsven, LC Kapitein - Journal of Cell Biology, 2020 - rupress.org
Cellular functioning relies on active transport of organelles by molecular motors. To explore
how intracellular organelle distributions affect cellular functions, several optogenetic …
how intracellular organelle distributions affect cellular functions, several optogenetic …
Condensate functionalization with microtubule motors directs their nucleation in space and allows manipulating RNA localization
A Cochard, A Safieddine, P Combe… - The EMBO …, 2023 - embopress.org
The localization of RNAs in cells is critical for many cellular processes. Whereas motor‐
driven transport of ribonucleoprotein (RNP) condensates plays a prominent role in RNA …
driven transport of ribonucleoprotein (RNP) condensates plays a prominent role in RNA …
A novel strategy to visualize vesicle‐bound kinesins reveals the diversity of kinesin‐mediated transport
R Yang, Z Bostick, A Garbouchian, J Luisi, G Banker… - Traffic, 2019 - Wiley Online Library
In mammals, 15 to 20 kinesins are thought to mediate vesicle transport. Little is known about
the identity of vesicles moved by each kinesin or the functional significance of such diversity …
the identity of vesicles moved by each kinesin or the functional significance of such diversity …
Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium
ÖZ Aydin, SO Taflan, C Gurkaslar… - PLoS biology, 2020 - journals.plos.org
Centriolar satellites are dynamic, membraneless granules composed of over 200 proteins.
They store, modify, and traffic centrosome and primary cilium proteins, and help to regulate …
They store, modify, and traffic centrosome and primary cilium proteins, and help to regulate …
Topology and function of translocated EspZ
EspZ and Tir are essential virulence effectors of enteropathogenic Escherichia coli (EPEC).
EspZ, the second translocated effector, has been suggested to antagonize host cell death …
EspZ, the second translocated effector, has been suggested to antagonize host cell death …