Recent advances in biological single-molecule applications of optical tweezers and fluorescence microscopy

MH Shabestari, AEC Meijering, WH Roos… - Methods in …, 2017 - Elsevier
Over the past two decades, single-molecule techniques have evolved into robust tools to
study many fundamental biological processes. The combination of optical tweezers with …

Recent Advances of Optical Tweezers–Based Dynamic Force Spectroscopy and Mechanical Measurement Assays for Live-Cell Mechanobiology

H Wang, Y Guo, R Zou, H Hu, Y Wang, F Wang… - Frontiers in …, 2022 - frontiersin.org
Cells sense and respond to mechanical stimuli for activation, proliferation, migration, and
differentiation. The associated mechanosensing and biomechanical properties of cells and …

Biophotonics

G Keiser - Springer. doi, 2016 - Springer
The discipline of biophotonics or biomedical optics has undergone a fascinating journey in
the past several decades, and it is still growing rapidly. As the name biophotonics implies …

Pitch-rotational manipulation of single cells and particles using single-beam thermo-optical tweezers

S Kumar, M Gunaseelan, R Vaippully… - Biomedical Optics …, 2020 - opg.optica.org
3D pitch rotation of microparticles and cells assumes importance in a wide variety of
applications in biology, physics, chemistry and medicine. Applications such as cell imaging …

Multiview microscopy of single cells through microstructure-based indirect optical manipulation

G Vizsnyiczai, A Búzás… - Biomedical optics …, 2020 - opg.optica.org
Fluorescent observation of cells generally suffers from the limited axial resolution due to the
elongated point spread function of the microscope optics. Consequently, three-dimensional …

Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells

BL Aekbote, T Fekete, J Jacak, G Vizsnyiczai… - Biomedical optics …, 2015 - opg.optica.org
We introduce a method that combines two-photon polymerization (TPP) and surface
functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D …

Single Cell Reactomics: Real‐Time Single‐Cell Activation Kinetics of Optically Trapped Macrophages

GF Vasse, P Buzón, BN Melgert, WH Roos… - Small …, 2021 - Wiley Online Library
Macrophages are well known for their role in immune responses and tissue homeostasis.
They can polarize towards various phenotypes in response to biophysical and biochemical …

Modern trends in biophotonics for clinical diagnosis and therapy to solve unmet clinical needs

C Krafft - Journal of biophotonics, 2016 - Wiley Online Library
This contribution covers recent original research papers in the biophotonics field. The
content is organized into main techniques such as multiphoton microscopy, Raman …

In Singulo Biophysics: accessing the Dynamics of Intracellular Processes at the Molecular and Cellular Levels

PB Rodriguez - 2022 - research.rug.nl
The understanding of the mechanisms behind the dynamics of biological processes is
crucial to build a more comprehensive picture of the principles governing living systems. At …

Macrophage-matrix interactions: orchestrating the fibrotic response?

G Vasse - 2021 - research.rug.nl
Even though years of research have advanced our understanding of idiopathic pulmonary
fibrosis (IPF), the expanded knowledge on risk factors and mechanisms has not yet resulted …