Application of flow cytometry to industrial microbial bioprocesses
Flow cytometry (FC) is a powerful technique for the rapid analysis of single cells in a mixture,
by means of light-scattering and fluorescence measurements. In biotechnology, the power of …
by means of light-scattering and fluorescence measurements. In biotechnology, the power of …
Flow cytometry in biotechnology
M Rieseberg, C Kasper, KF Reardon… - Applied microbiology and …, 2001 - Springer
Flow cytometry is a general method for rapidly analyzing large numbers of cells individually
using light-scattering, fluorescence, and absorbence measurements. The power of this …
using light-scattering, fluorescence, and absorbence measurements. The power of this …
System-wide perturbation analysis with nearly complete coverage of the yeast proteome by single-shot ultra HPLC runs on a bench top Orbitrap
Yeast remains an important model for systems biology and for evaluating proteomics
strategies. In-depth shotgun proteomics studies have reached nearly comprehensive …
strategies. In-depth shotgun proteomics studies have reached nearly comprehensive …
Analytical distributions for detailed models of stochastic gene expression in eukaryotic cells
The stochasticity of gene expression presents significant challenges to the modeling of
genetic networks. A two-state model describing promoter switching, transcription, and …
genetic networks. A two-state model describing promoter switching, transcription, and …
Continuous inertial focusing and separation of particles by shape
An effective approach to separating shaped particles is needed to isolate disease-causing
cells for diagnostics or to aid in purifying nonspherical particles in applications ranging from …
cells for diagnostics or to aid in purifying nonspherical particles in applications ranging from …
Molecular genetics of Schizosaccharomyces pombe
SA Sabatinos, SL Forsburg - Methods in enzymology, 2010 - Elsevier
In this chapter we present basic protocols for the use of Schizosaccharomyces pombe,
commonly known as fission yeast, in molecular biology and genetics research. Fission yeast …
commonly known as fission yeast, in molecular biology and genetics research. Fission yeast …
Expression homeostasis during DNA replication
Genome replication introduces a stepwise increase in the DNA template available for
transcription. Genes replicated early in S phase experience this increase before late …
transcription. Genes replicated early in S phase experience this increase before late …
Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus
R Azzam, SL Chen, W Shou, AS Mah, G Alexandru… - Science, 2004 - science.org
Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive
state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 …
state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 …
Separation and Enrichment of Yeast Saccharomyces cerevisiae by Shape Using Viscoelastic Microfluidics
Yeast Saccharomyces cerevisiae (S. Cerevisiae) is one of the most attractive microbial
species used for industrial production of value-added products and is an important model …
species used for industrial production of value-added products and is an important model …
Cell cycle synchronization of tobacco BY-2 cells
F Kumagai-Sano, T Hayashi, T Sano, S Hasezawa - Nature protocols, 2006 - nature.com
Synchronization is a powerful technique for understanding cell cycle events. Here, we
describe the procedure for synchronizing tobacco bright yellow 2 (BY-2) cell line, with which …
describe the procedure for synchronizing tobacco bright yellow 2 (BY-2) cell line, with which …