Challenges facing CRISPR/Cas9-based genome editing in plants

S Son, SR Park - Frontiers in plant science, 2022 - frontiersin.org
The development of plant varieties with desired traits is imperative to ensure future food
security. The revolution of genome editing technologies based on the clustered regularly …

Genome editing around the globe: An update on policies and perceptions

T Sprink, R Wilhelm, F Hartung - Plant Physiology, 2022 - academic.oup.com
A decade ago, the CRISPR/Cas system has been adapted for genome editing. Since then,
hundreds of organisms have been altered using genome editing and discussions were …

The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model

Y Li, J Xu, X Guo, Z Li, L Cao, S Liu, Y Guo, G Wang… - Genome biology, 2023 - Springer
Abstract Background The CRISPR-Cas13 system is an RNA-guided RNA-targeting system
and has been widely used in transcriptome engineering with potentially important clinical …

Occurrence and nature of off-target modifications by CRISPR-Cas genome editing in plants

MHJ Sturme, JP van der Berg… - … agricultural science & …, 2022 - ACS Publications
CRISPR-Cas-based genome editing allows for precise and targeted genetic modification of
plants. Nevertheless, unintended off-target edits can arise that might confer risks when …

Accelerating wood domestication in forest trees through genome editing: Advances and prospects

C Anders, L Hoengenaert, W Boerjan - Current opinion in plant biology, 2023 - Elsevier
The high economic value of wood requires intensive breeding towards multipurpose
biomass. However, long breeding cycles hamper the fast development of novel tree varieties …

Genome centric engineering using ZFNs, TALENs and CRISPR-Cas9 systems for trait improvement and disease control in Animals

AK Wani, N Akhtar, R Singh, A Prakash… - Veterinary research …, 2023 - Springer
Livestock is an essential life commodity in modern agriculture involving breeding and
maintenance. The farming practices have evolved mainly over the last century for …

CRISPR/Cas9-mediated multiplex gene editing of gamma and omega gliadins: paving the way for gliadin-free wheat

S Sánchez-León, M Marín-Sanz… - Journal of …, 2024 - academic.oup.com
Wheat is a staple cereal in the human diet. Despite its significance, an increasing
percentage of the population suffers adverse reactions to wheat, which are triggered by …

Genome editing for improving crop nutrition

A Nagamine, H Ezura - Frontiers in Genome Editing, 2022 - frontiersin.org
Genome editing technologies, including CRISPR/Cas9 and TALEN, are excellent genetic
modification techniques and are being proven to be powerful tools not only in the field of …

Gene editing for plant resistance to abiotic factors: a systematic review

FS Nascimento, AJ Rocha, JMS Soares… - Plants, 2023 - mdpi.com
Agricultural crops are exposed to various abiotic stresses, such as salinity, water deficits,
temperature extremes, floods, radiation, and metal toxicity. To overcome these challenges …

Unintended genomic outcomes in current and next generation GM techniques: A systematic review

P Chu, SZ Agapito-Tenfen - Plants, 2022 - mdpi.com
Classical genetic engineering and new genome editing techniques, especially the
CRISPR/Cas technology, increase the possibilities for modifying the genetic material in …