Quantitative proteomics using SILAC: Principles, applications, and developments

X Chen, S Wei, Y Ji, X Guo, F Yang - Proteomics, 2015 - Wiley Online Library
SILAC is based on direct addition of selected stable isotope amino acids into the cell culture
medium, allowing superior quantitative analysis of the cellular proteome compared to other …

Recent advances in glycoproteomic analysis by mass spectrometry

S Suttapitugsakul, F Sun, R Wu - Analytical chemistry, 2019 - ACS Publications
Glycosylation is one of the most common protein modifications and is essential for cells. This
modification is exceptionally complex because glycans are highly diverse and can be …

Biomimetic proteolipid vesicles for targeting inflamed tissues

R Molinaro, C Corbo, JO Martinez, F Taraballi… - Nature materials, 2016 - nature.com
A multitude of micro-and nanoparticles have been developed to improve the delivery of
systemically administered pharmaceuticals, which are subject to a number of biological …

Anti-human CD73 monoclonal antibody inhibits metastasis formation in human breast cancer by inducing clustering and internalization of CD73 expressed on the …

MG Terp, KA Olesen, EC Arnspang… - The Journal of …, 2013 - journals.aai.org
Recent studies have shown that Abs that target the cell-surface enzyme CD73 (ecto-5′-
nucleotidase) reduce growth of primary tumors and metastasis in syngenic mice by inhibiting …

Plasma membrane proteomics and its application in clinical cancer biomarker discovery

R Leth-Larsen, RR Lund, HJ Ditzel - Molecular & Cellular Proteomics, 2010 - ASBMB
Plasma membrane proteins that are exposed on the cell surface have important biological
functions, such as signaling into and out of the cells, ion transport, and cell-cell and cell …

Integral membrane proteins in proteomics. How to break open the black box?

O Vit, J Petrak - Journal of Proteomics, 2017 - Elsevier
Integral membrane proteins (IMPs) are coded by 20–30% of human genes and execute
important functions–transmembrane transport, signal transduction, cell-cell communication …

From bottom-up to cell surface proteomics: detergents or no detergents, that is the question

Z Brough, Z Zhao… - Biochemical Society …, 2024 - portlandpress.com
Measuring the expression levels of membrane proteins (MPs) is crucial for understanding
cell differentiation and tissue specificity, defining disease characteristics, identifying …

Biomarker sensing platforms based on fluorescent metal nanoclusters

MV Romeo, E López-Martínez… - Nanoscale …, 2021 - pubs.rsc.org
Metal nanoclusters (NCs) and their unique properties are increasing in importance and their
applications are covering a wide range of areas. Their remarkable fluorescence properties …

Isolation of cell surface proteins for mass spectrometry-based proteomics

S Elschenbroich, Y Kim, JA Medin… - Expert review of …, 2010 - Taylor & Francis
Defining the cell surface proteome has profound importance for understanding cell
differentiation and cell–cell interactions, as well as numerous pathogenic abnormalities …

A proteomics and transcriptomics approach to identify leukemic stem cell (LSC) markers

F Bonardi, F Fusetti, P Deelen, D van Gosliga… - Molecular & Cellular …, 2013 - ASBMB
Interactions between hematopoietic stem cells and their niche are mediated by proteins
within the plasma membrane (PM) and changes in these interactions might alter …