Serotype-agnostic affinity purification of adeno-associated virus (AAV) via peptide-functionalized chromatographic resins

S Shastry, E Barbieri, A Minzoni, W Chu… - … of Chromatography A, 2024 - Elsevier
Adeno-associated viruses (AAVs) have emerged as a prominent family of vectors for gene
delivery, providing therapeutic options to diseases once deemed incurable. At the same …

Chromatography in Downstream Processing of Recombinant Adeno‐Associated Viruses: A Review of Current and Future Practises

JK Lorek, M Isaksson, B Nilsson - Biotechnology and …, 2025 - Wiley Online Library
Recombinant adeno‐associated virus (rAAV) has emerged as an attractive gene delivery
vector platform to treat both rare and pervasive diseases. With more and more rAAV‐based …

AAV Capsid Modification and Its Influence on Viral Protein Stoichiometry and Packaging Fitness: Current Understandings and Future Direction

DM Dogbey, S Barth - Molecular Biotechnology, 2025 - Springer
The field of gene therapy has witnessed significant advancements in the utilization of Adeno-
associated virus (AAV) owing to its inherent biological advantages. Targeted AAV vectors …

Mechanistic modeling of empty‐full separation in recombinant adeno‐associated virus production using anion‐exchange membrane chromatography

J Gomis‐Fons, B Zee, D Hurwit, J Woo… - Biotechnology and …, 2024 - Wiley Online Library
Recombinant adeno‐associated viral vectors (rAAVs) have become an industry‐standard
technology in the field of gene therapy, but there are still challenges to be addressed in their …

[HTML][HTML] Structural Changes Likely Cause Chemical Differences between Empty and Full AAV Capsids

CL Heldt, MA Skinner, GS Anand - Biomedicines, 2024 - mdpi.com
Due to the success of adeno associated viruses (AAVs) in treating single-gene diseases,
improved manufacturing technology is now needed to meet their demand. The largest …

Studying the ssDNA loaded adeno-associated virus aggregation using coarse-grained molecular dynamics simulations

T Duran, S Naik, L Sharifi, WR DiLuzio… - International Journal of …, 2024 - Elsevier
The aggregation of adeno-associated viral (AAV) capsids in an aqueous environment was
investigated via coarse-grained molecular dynamics (CG-MD) simulations. The primary …

DNA Released by Adeno-Associated Virus Strongly Alters Capsid Aggregation Kinetics in a Physiological Solution

CW Jarand, K Baker, M Petroff, M Jin… - …, 2024 - ACS Publications
While adeno-associated virus is a leading vector for gene therapy, significant gaps remain in
understanding AAV degradation and stability. In this work, we study the degradation of an …

Separation of full, empty, and partial adeno-associated virus capsids via anion-exchange chromatography with continuous recycling and accumulation

YS Lee, J Lee, K Fang, GV Gee, B Rogers… - … of Chromatography B, 2024 - Elsevier
The field of recombinant adeno-associated virus (rAAV) gene therapy has attracted
increasing attention over decades. Within the ongoing challenges of rAAV manufacturing …

[HTML][HTML] Purification of AAV8 through a scalable two-step monolithic chromatography approach

A Nascimento, TQ Faria, F Bollmann… - … of Chromatography A, 2025 - Elsevier
Adeno-associated viruses (AAV) are becoming increasingly popular as a powerful tool for
gene delivery therapy applications. Although processes to produce AAV are established …

Comparative Analysis of Empty and Full Adeno-Associated Viruses under Stressed Conditions by Anion-Exchange Chromatography, Analytical Ultracentrifugation …

JA Townsend, S Li, L Sweezy, N Liu… - Journal of …, 2025 - Elsevier
Adeno-associated viruses (AAVs) have emerged as a promising gene delivery vehicle for
the treatment of diseases. As AAVs are a complex therapeutic modality, new analytical …