The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …

Synthetic Biology is a rapidly growing interdisciplinary field that is primarily built upon
foundational advances in molecular biology combined with engineering design principles …

Modular cloning has become a benchmark technology in synthetic biology. However, a
notable disparity exists between its remarkable development and the need for …

Synthetic biology relies on the manufacture of large and complex DNA constructs from
libraries of genetic parts. Golden Gate and other Type IIS restriction enzyme-dependent …

The industrial yeast Komagataella phaffii (formerly named Pichia pastoris) is commonly
used to synthesize recombinant proteins, many of which are used as human therapeutics or …

Fungal unspecific peroxygenases (UPOs) represent an enzyme class catalysing versatile
oxyfunctionalisation reactions on a broad substrate scope. They are occurring as secreted …

The ability to engineer biological systems, whether to introduce novel functionality or
improved performance, is a cornerstone of biotechnology and synthetic biology. Typically …

Site-directed methods for the generation of genetic diversity are essential tools in the field of
directed enzyme evolution. The Golden Gate cloning technique has been proven to be an …

With the rise of new DNA part libraries and technologies for assembling DNA, synthetic
biologists are increasingly constructing and screening combinatorial libraries to optimize …

Plant synthetic biology is a fast-evolving field that employs engineering principles to
empower research and bioproduction in plant systems. Nevertheless, in the whole synthetic …