Programmable gene-editing tools have transformed the life sciences and have shown
potential for the treatment of genetic disease. Among the CRISPR–Cas technologies that …

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases …

Realizing the promise of prime editing for the study and treatment of genetic disorders
requires efficient methods for delivering prime editors (PEs) in vivo. Here we describe the …

CRISPR-based drugs can theoretically manipulate any genetic target. In practice, however,
these drugs must enter the desired cell without eliciting an unwanted immune response, so …

Prime editing (PE) enables precise and versatile genome editing without requiring double-
stranded DNA breaks. Here we describe the systematic optimization of PE systems to …

Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …

The editing efficiencies of prime editing (PE) using ribonucleoprotein (RNP) and RNA
delivery are not optimal due to the challenges in solid-phase synthesis of long PE guide …

Directed protein evolution is central to biomedical applications but faces challenges like
experimental complexity, inefficient multi-property optimization, and local maxima traps …

Genome editing with prime editors based on CRISPR-Cas9 is limited by the large size of the
system and the requirement for a G/C-rich protospacer-adjacent motif (PAM) sequence …