As genome-editing nucleases move toward broader clinical applications, the need to define
the limits of their specificity and efficiency increases. A variety of approaches for nuclease …

Human immunodeficiency virus (HIV) infections and HIV-induced acquired
immunodeficiency syndrome (AIDS) continue to represent a global health burden. There is …

CRISPR/Cas complexes enable precise gene editing in a wide variety of organisms. While
the rigid identification of DNA sequences by these systems minimizes the potential for off …

Background A barrier to HIV-1 cure rests in the persistence of proviral DNA in infected CD4+
leukocytes. The high HIV-1 mutation rate leads to viral diversity, immune evasion, and …

The CRISPR-Cas9 system has been used for genome editing of various organisms. We
reported inhibition of the human immunodeficiency virus (HIV) in cell culture infections with a …

Abstract Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)‐Cas
technology possesses revolutionary potential to positively affect various domains of drug …

Highlights•HIV provirus in infected cells can be inactivated with CRISPR–
Cas9.•Combinatorial CRISPR–Cas9 attack can prevent viral escape.•Both mutation and …

Gene editing may be used to excise the human immunodeficiency virus type 1 (HIV-1)
provirus from the host cell genome, possibly eradicating the infection. Here, using cells …

Introduction Antiretroviral therapy (ART) has transformed prognoses for HIV-1-infected
individuals but requires lifelong adherence to prevent viral resurgence. Targeted elimination …

Although several studies demonstrated that the HIV proviral DNA can be effectively targeted
and inactivated by the CRISPR-Cas9 system, the precise inactivation mechanism has not …