Point of care diagnostics: status and future

V Gubala, LF Harris, AJ Ricco, MX Tan… - Analytical …, 2012 - ACS Publications
We review point-of-care (POC) diagnostics, in vitro diagnostic (IVD) tests that do not involve
the use of laboratory staff and facilities to provide the result. The analytical “targets” include …

Dynamic computer simulations of electrophoresis: 2010–2020

W Thormann, RA Mosher - Electrophoresis, 2022 - Wiley Online Library
The transport of components in liquid media under the influence of an applied electric field
can be described with the continuity equation. It represents a nonlinear conservation law …

Instabilities of the pH gradient in carrier ampholyte‐based isoelectric focusing: elucidation of the contributing electrokinetic processes by computer simulation

W Thormann, RA Mosher - Electrophoresis, 2021 - Wiley Online Library
Electrokinetic processes that lead to pH gradient instabilities in carrier ampholyte‐based IEF
are reviewed. In addition to electroosmosis, there are four of electrophoretic nature, namely …

Microfluidic isoelectric focusing of amyloid beta peptides followed by micropillar-matrix-assisted laser desorption ionization-mass spectrometry

S Mikkonen, J Jacksén, J Roeraade… - Analytical …, 2016 - ACS Publications
A novel method for preconcentration and purification of the Alzheimer's disease related
amyloid beta (Aβ) peptides by isoelectric focusing (IEF) in 75 nL microchannels combined …

Droplet-based in situ compartmentalization of chemically separated components after isoelectric focusing in a Slipchip

Y Zhao, F Pereira, AJ deMello, H Morgan, X Niu - Lab on a Chip, 2014 - pubs.rsc.org
Isoelectric focusing (IEF) is a powerful and widely used technique for protein separation and
purification. There are many embodiments of microscale IEF that use capillary or microfluidic …

A photoinduced nanoparticle separation in microchannels via ph-sensitive surface traps

M Ebara, JM Hoffman, AS Hoffman, PS Stayton, JJ Lai - Langmuir, 2013 - ACS Publications
A microfluidic surface trap was developed for capturing pH-sensitive nanoparticles via a
photoinitiated proton-releasing reaction of o-nitrobenzaldehyde (o-NBA) that reduces the …

A novel microscale preparative gel electrophoresis system

F Hao, J Li, R Zhai, F Jiao, Y Zhang, X Qian - Analyst, 2016 - pubs.rsc.org
Currently, the separation targets of preparative electrophoresis range from milligrams to
micrograms of proteins. However, most commercially available preparative electrophoretic …

毛细管电泳在线聚焦原理, 技术及应用

刘胜权, 汪海林 - 色谱, 2011 - chrom-china.com
毛细管电泳以其分析速度快, 分离效率高, 操作简便, 能够实现高通量而获得了广泛的应用,
但由于检测窗口小而导致其检测灵敏度低. 为了提高检测灵敏度, 目前已发展了多种毛细管电泳 …

Computer simulations of sample preconcentration in carrier‐free systems and isoelectric focusing in microchannels using simple ampholytes

S Mikkonen, W Thormann, Å Emmer - Electrophoresis, 2015 - Wiley Online Library
In this work, electrophoretic preconcentration of protein and peptide samples in
microchannels was studied theoretically using the 1D dynamic simulator GENTRANS, and …

A brief review of other notable electrophoretic methods

JD Fesmire - Electrophoretic separation of proteins: methods and …, 2019 - Springer
Electrophoretic methodologies for qualitative and preparative purposes are commonly used
in biological research and have been well established as an integral analytical tool for a …