Design and application of fluorescent probes to detect cellular physical microenvironments

J Ma, R Sun, K Xia, Q Xia, Y Liu, X Zhang - Chemical Reviews, 2024 - ACS Publications
The microenvironment is indispensable for functionality of various biomacromolecules,
subcellular compartments, living cells, and organisms. In particular, physical properties …

Advanced organic optoelectronic materials: Harnessing excited‐state intramolecular proton transfer (ESIPT) process

JE Kwon, SY Park - Advanced Materials, 2011 - Wiley Online Library
Recently, organic fluorescent molecules harnessing the excited‐state intramolecular proton
transfer (ESIPT) process are drawing great attention due to their unique photophysical …

Excited-state proton coupled charge transfer modulated by molecular structure and media polarization

AP Demchenko, KC Tang, PT Chou - Chemical Society Reviews, 2013 - pubs.rsc.org
Charge and proton transfer reactions in the excited states of organic dyes can be coupled in
many different ways. Despite the complementarity of charges, they can occur on different …

Fluorescent probes for lipid rafts: from model membranes to living cells

AS Klymchenko, R Kreder - Chemistry & biology, 2014 - cell.com
Membrane microdomains (rafts) remain one of the controversial issues in biophysics.
Fluorescent molecular probes, which make these lipid nanostructures visible through optical …

[图书][B] Principles of fluorescence spectroscopy

JR Lakowicz - 2006 - Springer
The success of fluorescence experiments requires attention to experimental details and an
understanding of the instrumentation. There are also many potential artifacts that can distort …

[图书][B] Introduction to Fluorescence Sensing: Volume 2: Target Recognition and Imaging

AP Demchenko - 2023 - books.google.com
Fluorescence is the most popular technique in chemical and biological sensing because of
its ultimate sensitivity, high temporal and spatial resolution and versatility that enables …

Switchable nile red-based probe for cholesterol and lipid order at the outer leaflet of biomembranes

OA Kucherak, S Oncul, Z Darwich… - Journal of the …, 2010 - ACS Publications
Cholesterol and sphingomyelin form together a highly ordered membrane phase, which is
believed to play important biological functions in plasma membranes of mammalian cells …

Monitoring biophysical properties of lipid membranes by environment-sensitive fluorescent probes

AP Demchenko, Y Mély, G Duportail, AS Klymchenko - Biophysical journal, 2009 - cell.com
We review the main trends in the development of fluorescence probes to obtain information
about the structure, dynamics, and interactions in biomembranes. These probes are efficient …

Fluorescent biomembrane probe for ratiometric detection of apoptosis

VV Shynkar, AS Klymchenko… - Journal of the …, 2007 - ACS Publications
Herein, we developed the first ratiometric fluorescent probe for apoptosis detection. This
probe incorporates selectively into the outer leaflet of the cell plasma membrane and senses …

Multiparametric probing of intermolecular interactions with fluorescent dye exhibiting excited state intramolecular proton transfer

AS Klymchenko, AP Demchenko - Physical Chemistry Chemical …, 2003 - pubs.rsc.org
Excited-state intramolecular proton transfer (ESIPT) in 3-hydroxyflavone dyes allows us to
record, in addition to common spectroscopic parameters, the positions of absorption (νabs) …