Bacteria with streamlined genomes, that harbor full functional genes for essential metabolic
networks, are able to synthesize the desired products more effectively and thus have …

Bacterial cells are widely used to produce value-added products due to their versatility, ease
of manipulation, and the abundance of genome engineering tools. However, the efficiency of …

Site-specific recombinases such as the Cre-LoxP system are routinely used for genome
engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the …

Essential genes encode the processes that are necessary for life. Until recently, commonly
applied binary classifications left no space between essential and non-essential genes. In …

Mycoplasmas are successful pathogens that cause debilitating diseases in humans and
various animal hosts. Despite the exceptionally streamlined genomes, mycoplasmas have …

Yarrowia lipolytica is a non-conventional oleaginous yeast with great potential for industrial
production. Y. lipolytica has a high propensity for flux through tricarboxylic acid cycle …

Identifying open reading frames (ORFs) being translated is not a trivial task. ProTInSeq is a
technique designed to characterize proteomes by sequencing transposon insertions …

Genome reduction has been emerged as a powerful tool to construct ideal chassis for
synthetic biology. Random genome reduction couple genomic deletion with growth and has …

Bacteria are highly adaptable and grow in diverse niches, where they often interact with
eukaryotic organisms. These interactions with different hosts span the entire spectrum from …

Key message A large-effect QTL was fine mapped, which revealed 79 gene models, with 10
promising candidate genes, along with a novel inversion. Abstract In commercial maize …