Polymeric materials produced from fossil fuels have been intimately linked to the
development of industrial activities in the 20th century and, consequently, to the …

Single-cell RNA sequencing (scRNA-seq) technologies allow the dissection of gene
expression at single-cell resolution, which greatly revolutionizes transcriptomic studies. A …

In recent years, RNA-sequencing (RNA-seq) has emerged as a powerful technology for
transcriptome profiling. For a given gene, the number of mapped reads is not only …

Mammalian cells have about 30,000 times as many protein molecules as mRNA molecules,
which has major implications in the development of proteomics technologies. We discuss …

RNA-seq is currently considered the most powerful, robust and adaptable technique for
measuring gene expression and transcription activation at genome-wide level. As the …

The correct identification of differentially expressed genes (DEGs) between specific
conditions is a key in the understanding phenotypic variation. High-throughput transcriptome …

Plastic wastes are becoming the most common form of marine debris and present a growing
global pollution problem. Here, we used a screening approach on hundreds of plastic waste …

Abstract RNA-sequencing (RNA-seq) has a wide variety of applications, but no single
analysis pipeline can be used in all cases. We review all of the major steps in RNA-seq data …

HISAT (hierarchical indexing for spliced alignment of transcripts) is a highly efficient system
for aligning reads from RNA sequencing experiments. HISAT uses an indexing scheme …

Methods used to sequence the transcriptome often produce more than 200 million short
sequences. We introduce StringTie, a computational method that applies a network flow …