An efficient peptide ligase engineered from a bamboo asparaginyl endopeptidase

XB Wang, CH Zhang, T Zhang, HZ Li, YL Liu… - The FEBS …, 2024 - Wiley Online Library
In recent years, a few asparaginyl endopeptidases (AEPs) from certain higher plants have
been identified as efficient peptide ligases with wide applications in protein labeling and …

Preparing recombinant" Split AEP" for protein labeling.

M Ma, STM Tang, MT Dickerson, LYP Luk - Methods in enzymology, 2023 - europepmc.org
A variant originated from Oldenlandia affinis asparaginyl ligase, OaAEP1-C247A, has
emerged as an ideal tool for protein labeling. However, its preparation was laborious and …