A pan-cancer analysis of the prognostic and immunological role of β-actin (ACTB) in human cancers

Y Gu, S Tang, Z Wang, L Cai, H Lian, Y Shen… - …, 2021 - Taylor & Francis
ABSTRACT Beta-actin (ACTB), a highly conserved cytoskeleton structural protein, has been
regarded as a common housekeep gene and used as a reference gene for years. However …

Epigenetic immune cell counting in human blood samples for immunodiagnostics

U Baron, J Werner, K Schildknecht… - Science translational …, 2018 - science.org
Immune cell profiles provide valuable diagnostic information for hematologic and
immunologic diseases. Although it is the most widely applied analytical approach, flow …

[HTML][HTML] Total protein is an effective loading control for cerebrospinal fluid western blots

MA Collins, J An, D Peller, R Bowser - Journal of neuroscience methods, 2015 - Elsevier
Background Cerebrospinal fluid (CSF) has been used to identify biomarkers of neurological
disease. CSF protein biomarkers identified by high-throughput methods, however, require …

Transcriptional and metabolomic analysis of L-arginine/nitric oxide pathway in inflammatory bowel disease and its association with local inflammatory and angiogenic …

M Krzystek-Korpacka, M G. Fleszar… - International Journal of …, 2020 - mdpi.com
L-arginine/nitric oxide pathway in Crohn's disease (CD) and ulcerative colitis (UC) is poorly
investigated. The aim of current study is to quantify pathway serum metabolites in 52 CD (40 …

Mitochondrial calcium uniporter stabilization preserves energetic homeostasis during complex I impairment

E Balderas, DR Eberhardt, S Lee, JM Pleinis… - Nature …, 2022 - nature.com
Calcium entering mitochondria potently stimulates ATP synthesis. Increases in calcium
preserve energy synthesis in cardiomyopathies caused by mitochondrial dysfunction, and …

Screening and validation of the optimal panel of reference genes in colonic epithelium and relative cancer cell lines

Y Hu, Q Jiang, X Zhai, L Liu, Y Hong - Scientific Reports, 2023 - nature.com
Real-time quantitative polymerase chain reaction (RT-qPCR) is the most common method to
determine mRNA expression, and Minimum Information for Publication of RT-qPCR …

Long non-coding RNA GAS5 and intestinal MMP2 and MMP9 expression: a translational study in pediatric patients with IBD

M Lucafò, L Pugnetti, M Bramuzzo, D Curci… - International Journal of …, 2019 - mdpi.com
Background: The long non-coding RNA (lncRNA) growth arrest–specific transcript 5 (GAS5)
seems to be involved in the regulation of mediators of tissue injury, in particular matrix …

Validation of reference genes for normalization of relative qRT-PCR studies in papillary thyroid carcinoma

SA Razavi, M Afsharpad, MH Modarressi, M Zarkesh… - Scientific reports, 2019 - nature.com
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) in thyroid tumors
require accurate data normalization, however, there are no sufficient studies addressing the …

Oversecretion and overexpression of nicotinamide phosphoribosyltransferase/pre-B colony-enhancing factor/visfatin in inflammatory bowel disease reflects the …

K Neubauer, I Bednarz-Misa… - International Journal of …, 2019 - mdpi.com
Nicotinamide phosphoribosyltransferase's (Nampt) association with inflammatory bowel
disease (IBD) is unclear. The study was aimed at unraveling Nampt's clinical and diagnostic …

L-arginine/nitric oxide pathway is altered in colorectal cancer and can be modulated by novel derivatives from oxicam class of non-steroidal anti-inflammatory drugs

M Krzystek-Korpacka, B Szczęśniak-Sięga, I Szczuka… - Cancers, 2020 - mdpi.com
Simple Summary Nitric oxide and arginine metabolism in colorectal cancer (CRC) holds
potential for therapeutic intervention. We hypothesized that it can be modulated by oxicams …