Metabolic labeling of proteins with non-canonical amino acids (ncAAs) provides unique
bioorthogonal chemical groups during de novo synthesis by taking advantage of both …

Incorporation of unnatural amino acids (UNAAs) into proteins currently is an active biological
research area for various fundamental and applied science. In this context, cell-free …

Supplementing translation with noncanonical amino acids (ncAAs) can yield protein
sequences with new-to-nature functions but existing ncAA incorporation strategies suffer …

Recent advances in cell-free gene expression (CFE) systems have enabled their use for a
host of synthetic biology applications, particularly for rapid prototyping of genetic circuits and …

The site-specific incorporation of non-canonical amino acids (ncAAs) into proteins via amber
suppression provides access to novel protein properties, structures, and functions …

The genetic code of mammalian cells can be expanded to allow the incorporation of non-
canonical amino acids (ncAAs) by suppressing in-frame amber stop codons (UAG) with an …

Since its early development and use to decipher the genetic code, in vitro or 'cell-
free'systems have been used as an important research tool to understand biochemical …

Highlights•Cell-free chemical biosynthesis (CFCB) adds flexibility and control to
manufacturing.•CFCB enables modular assembly, flexible conditions, and direct access to …

Approximately one third of protein therapeutics are produced in Escherichia coli, targeting a
wide variety of diseases. However, due to immune recognition of endotoxin (a lipid …

Cell-free protein synthesis has emerged as a promising platform for the production of
therapeutic proteins due to its inherently open reaction environment, flexible reaction …