PCR technology for screening and quantification of genetically modified organisms (GMOs)

A Holst-Jensen, SB Rønning, A Løvseth… - Analytical and …, 2003 - Springer
Although PCR technology has obvious limitations, the potentially high degree of sensitivity
and specificity explains why it has been the first choice of most analytical laboratories …

Testing for genetically modified organisms (GMOs): Past, present and future perspectives

A Holst-Jensen - Biotechnology advances, 2009 - Elsevier
This paper presents an overview of GMO testing methodologies and how these have
evolved and may evolve in the next decade. Challenges and limitations for the application of …

Reliable detection and identification of genetically modified maize, soybean, and canola by multiplex PCR analysis

D James, A Schmidt, E Wall, M Green… - Journal of agricultural …, 2003 - ACS Publications
Multiplex PCR procedures were developed for simultaneously detecting multiple target
sequences in genetically modified (GM) soybean (Roundup Ready), maize (event 176, Bt11 …

Absence of detectable transgenes in local landraces of maize in Oaxaca, Mexico (2003–2004)

S Ortiz-García, E Ezcurra, B Schoel… - Proceedings of the …, 2005 - National Acad Sciences
In 2000, transgenes were detected in local maize varieties (landraces) in the mountains of
Oaxaca, Mexico [Quist, D. & Chapela, IH (2001) Nature 414, 541–543]. This region is part of …

Development of a seven-target multiplex PCR for the simultaneous detection of transgenic soybean and maize in feeds and foods

A Germini, A Zanetti, C Salati, S Rossi… - Journal of Agricultural …, 2004 - ACS Publications
The detection of genetically modified organisms (GMOs) in food and feed is an important
issue for all the subjects involved in raw material control, food industry, and distribution …

The Development and Standardization of Testing Methods for Genetically Modified Organisms and their Derived ProductsF

D Zhang, J Guo - Journal of Integrative Plant Biology, 2011 - Wiley Online Library
Abstract Dabing Zhang (Corresponding author) As the worldwide commercialization of
genetically modified organisms (GMOs) increases and consumers concern the safety of …

Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean usingnew reference molecules

Y Shindo, H Kuribara, T Matsuoka… - Journal of AOAC …, 2002 - academic.oup.com
Novel analytical methods based on real-time quantitative polymerase chain reactions by use
of new reference molecules were validated in interlaboratory studies for the quantitation of …

Applicability of the quantification of genetically modified organisms to foods processed from maize and soy

T Yoshimura, H Kuribara, T Matsuoka… - Journal of agricultural …, 2005 - ACS Publications
The applicability of quantifying genetically modified (GM) maize and soy to processed foods
was investigated using heat treatment processing models. The detection methods were …

[PDF][PDF] Transgene escape from GM crops and potential biosafety consequences: an environmental perspective

BR Lu - Collection of Biosafety reviews, 2008 - icgeb.org
The rapid progress of transgenic biotechnology has significantly promoted the development
and production of genetically modified (GM) crops. The extensive global cultivation of GM …

Development of a general method for detection and quantification of the P35S promoter based on assessment of existing methods

Y Wu, Y Wang, J Li, W Li, L Zhang, Y Li, X Li, J Li… - Scientific reports, 2014 - nature.com
Abstract The Cauliflower mosaic virus (CaMV) 35S promoter (P35S) is a commonly used
target for detection of genetically modified organisms (GMOs). There are currently 24 …