Genome replication involves dealing with obstacles that can result from DNA damage but
also from chromatin alterations, topological stress, tightly bound proteins or non-B DNA …

Accurate and complete replication of the genome in every cell division is a prerequisite of
genomic stability. Thus, both prokaryotic and eukaryotic replication forks are extremely …

Saccharomyces cerevisiae carries∼ 150 copies of rDNA in tandem repeats. It was found
that the absence of an essential subunit of RNA polymerase I (Pol I) in rpa135 deletion …

A cause of aging in yeast is the accumulation of circular species of ribosomal DNA (rDNA)
arising from the 100–200 tandemly repeated copies in the genome. We show here that …

Topoisomerase I cleavage complexes can be induced by a variety of DNA damages and by
the anticancer drug camptothecin. We have developed a ligation-mediated PCR (LM-PCR) …

Homologous recombination safeguards genome integrity, but it can also cause genome
instability of important consequences for cell proliferation and organism development …

We report that a highly purified human origin recognition complex (HsORC) has intrinsic
DNA-binding activity, and that this activity is modestly stimulated by ATP. HsORC binds …

Background: In the rRNA gene cluster on the Saccharomyces cerevisiae chromosome XII, a
unique site that blocks progression of the replication fork exists in a single unit of the …

The mechanisms by which metazoan origins of DNA replication are defined, regulated, and
influenced by chromosomal events remain poorly understood. To gain insights into these …

It is known that mutations in gene SIR2 increase and those in FOB1 decrease recombination
within rDNA repeats as assayed by marker loss or extrachromosomal rDNA circle formation …