Prokaryotic Argonautes (pAgos) are an emerging class of programmable endonucleases
that are believed to be more flexible than existing CRISPR–Cas systems and have …

Recognition and repression of RNA targets by Argonaute proteins guided by small RNAs is
the essence of RNA interference in eukaryotes. Argonaute proteins with diverse structures …

Argonaute proteins are programmable nucleases that are found in both eukaryotes and
prokaryotes and provide defense against invading genetic elements. Although some …

Argonaute (Ago) proteins are key players in RNA interference in eukaryotes, where they
function as RNA-guided RNA endonucleases. Prokaryotic Argonautes (pAgos) are much …

Programmable nucleases are the most important tool for manipulating the genes and
genomes of both prokaryotes and eukaryotes. Since the end of the 20th century, many …

Foodborne pathogens endanger human health. Therefore, sensitive and accurate detection
assays are key for their prevention and control. Microdroplet digital nucleic acid platforms …

Circulating tumor DNA (ctDNA) is a noninvasive biomarker for liquid biopsy with important
clinical and biological information, but existing detection techniques are expensive, complex …

Members of the conserved Argonaute (Ago) protein family provide defence against invading
nucleic acids in eukaryotes in the process of RNA interference. Many prokaryotes also …

Site-specific nucleases are crucial for genome engineering applications in medicine and
agriculture. The ideal site-specific nucleases are easily reprogrammable, highly specific in …

Abstract The DNA‐guided (gDNA) Argonaute from Thermus thermophilus (TtAgo) has little
potential for nucleic acid detection and gene editing due to its poor dsDNA cleavage activity …