Physical methods for genetic plant transformation

AL Rivera, M Gómez-Lim, F Fernández, AM Loske - Physics of life reviews, 2012 - Elsevier
Production of transgenic plants is a routine process for many crop species. Transgenes are
introduced into plants to confer novel traits such as improved nutritional qualities, tolerance …

Methodologies to increase the transformation efficiencies and the range of bacteria that can be transformed

TEV Aune, FL Aachmann - Applied microbiology and biotechnology, 2010 - Springer
Methods for transfer of exogenous DNA into cells are essential for genetics and molecular
biology, and the lack of effective methods hampers research on many different species of …

Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit …

T Xin, H Tian, Y Ma, S Wang, L Yang, X Li… - Horticulture …, 2022 - academic.oup.com
Fruits and vegetables in the Cucurbitaceae family, such as cucumber, melon, watermelon,
and squash, contribute greatly to the human diet. The widespread use of genome editing …

Improved cotyledonary node method using an alternative explant derived from mature seed for efficient Agrobacterium-mediated soybean transformation

MM Paz, JC Martinez, AB Kalvig, TM Fonger, K Wang - Plant cell reports, 2006 - Springer
The utility of transformation for soybean improvement requires an efficient system for
production of stable transgenic lines. We describe here an improved cotyledonary node …

Assessment of conditions affecting Agrobacterium-mediated soybean transformation using the cotyledonary node explant

MM Paz, H Shou, Z Guo, Z Zhang, AK Banerjee… - Euphytica, 2004 - Springer
Conditions affecting Agrobacterium-mediated transformation of soybean [Glycine max (L.)
Merr.], including seed vigor of explant source, selection system, and cocultivation conditions …

L-Cysteine increases Agrobacterium-mediated T-DNA delivery into soybean cotyledonary-node cells

P Olhoft, D Somers - Plant Cell Reports, 2001 - Springer
A major limitation in producing transgenic soybeans [Glycine max (L.) Merrill] using the
Agrobacterium-mediated cotyledonary-node method is low-frequency T-DNA transfer from …

A Rapid, Highly Efficient and Economical Method of Agrobacterium-Mediated In planta Transient Transformation in Living Onion Epidermis

K Xu, X Huang, M Wu, Y Wang, Y Chang, K Liu… - PLoS …, 2014 - journals.plos.org
Transient transformation is simpler, more efficient and economical in analyzing protein
subcellular localization than stable transformation. Fluorescent fusion proteins were often …

Plant‐based vaccines: Production and challenges

E Laere, APK Ling, YP Wong, RY Koh… - Journal of …, 2016 - Wiley Online Library
Plant‐based vaccine technologies involve the integration of the desired genes encoding the
antigen protein for specific disease into the genome of plant tissues by various methods …

Edit at will: genotype independent plant transformation in the era of advanced genomics and genome editing

AP Kausch, K Nelson-Vasilchik, J Hague, M Mookkan… - Plant Science, 2019 - Elsevier
The combination of advanced genomics, genome editing and plant transformation biology
presents a powerful platform for basic plant research and crop improvement. Together these …

Ultrasound: mechanical gene transfer into plant cells by sonoporation

Y Liu, H Yang, A Sakanishi - Biotechnology advances, 2006 - Elsevier
Development of nonviral gene transfer methods would be a valuable alternative of gene
therapy or transformation. Ultrasound can produce a variety of nonthermal bioeffects via …