Integrating multiple 'omics' analysis for microbial biology: application and methodologies

W Zhang, F Li, L Nie - Microbiology, 2010 - microbiologyresearch.org
Recent advances in various 'omics' technologies enable quantitative monitoring of the
abundance of various biological molecules in a high-throughput manner, and thus allow …

CE‐MS in metabolomics

R Ramautar, GW Somsen, GJ de Jong - Electrophoresis, 2009 - Wiley Online Library
An overview of the use of CE‐MS in the field of metabolomics is provided. Metabolomics is
concerned with the comprehensive analysis of endogenous low‐molecular‐weight …

Systematic phenome analysis of Escherichia coli multiple‐knockout mutants reveals hidden reactions in central carbon metabolism

K Nakahigashi, Y Toya, N Ishii, T Soga… - Molecular systems …, 2009 - embopress.org
Central carbon metabolism is a basic and exhaustively analyzed pathway. However, the
intrinsic robustness of the pathway might still conceal uncharacterized reactions. To test this …

Metabolic flux network and analysis of fermentative hydrogen production

G Cai, B Jin, P Monis, C Saint - Biotechnology advances, 2011 - Elsevier
Fermentative hydrogen production (FHP) has received a great R & D interest in recent
decades, as it offers a potential means of producing H2 from a variety of renewable …

13C metabolic flux analysis in complex systems

N Zamboni - Current opinion in biotechnology, 2011 - Elsevier
Experimental determination of in vivo metabolic rates by methods of 13C metabolic flux
analysis is a pivotal approach to unravel structure and regulation of metabolic networks, in …

Advances in analysis of microbial metabolic fluxes via 13C isotopic labeling

YJ Tang, HG Martin, S Myers… - Mass spectrometry …, 2009 - Wiley Online Library
Metabolic flux analysis via 13C labeling (13C MFA) quantitatively tracks metabolic pathway
activity and determines overall enzymatic function in cells. Three core techniques are …

Collisional fragmentation of central carbon metabolites in LC‐MS/MS increases precision of 13C metabolic flux analysis

M Rühl, B Rupp, K Nöh, W Wiechert… - Biotechnology and …, 2012 - Wiley Online Library
Experimental determination of fluxes by 13C‐tracers relies on detection of 13C‐patterns in
metabolites or by‐products. In the field of 13C metabolic flux analysis, the most recent …

Capillary electrophoresis in bioanalysis

V Kostal, J Katzenmeyer, EA Arriaga - Analytical chemistry, 2008 - ACS Publications
For the period January 2006 to December 2007, Web of Science reports∼ 4800 hits on
capillary electrophoresis (CE) including 410 reviews. Because of the prominence of CE …

13C‐metabolic flux analysis for batch culture of Escherichia coli and its pyk and pgi gene knockout mutants based on mass isotopomer distribution of intracellular …

Y Toya, N Ishii, K Nakahigashi, T Hirasawa… - Biotechnology …, 2010 - Wiley Online Library
Since most bio‐production processes are conducted in a batch or fed‐batch manner, the
evaluation of metabolism with respect to time is highly desirable. Toward this aim, we …

Capillary electrophoresis as a metabolomics tool for non-targeted fingerprinting of biological samples

C Barbas, EP Moraes, A Villasenor - Journal of pharmaceutical and …, 2011 - Elsevier
Metabolomics, understood as a data driven strategy trying to find markers of a situation
under study without a priori hypothesis, has rapidly caught the attention and evolved from …