Paramagnetic chemical probes for studying biological macromolecules

Q Miao, C Nitsche, H Orton, M Overhand… - Chemical …, 2022 - ACS Publications
Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR)
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …

Deuteration for high-resolution detection of protons in protein magic angle spinning (MAS) solid-state NMR

B Reif - Chemical Reviews, 2021 - ACS Publications
Proton detection developed in the last 20 years as the method of choice to study
biomolecules in the solid state. In perdeuterated proteins, proton dipolar interactions are …

The effect of disorder on endogenous MAS-DNP: Study of silicate glasses and crystals

B Thomas, D Jardón-Álvarez, R Carmieli… - The Journal of …, 2023 - ACS Publications
In dynamic nuclear polarization nuclear magnetic resonance (DNP-NMR) experiments, the
large Boltzmann polarization of unpaired electrons is transferred to surrounding nuclei …

Two decades of progress in structural and dynamic studies of amyloids by solid-state NMR

CP Jaroniec - Journal of Magnetic Resonance, 2019 - Elsevier
In this perspective article I briefly highlight the rapid progress made over the past two
decades in atomic level structural and dynamic studies of amyloids, which are …

Structural studies of amyloid fibrils by paramagnetic solid-state nuclear magnetic resonance spectroscopy

T Theint, Y Xia, PS Nadaud… - Journal of the …, 2018 - ACS Publications
Application of paramagnetic solid-state NMR to amyloids is demonstrated, using Y145Stop
human prion protein modified with nitroxide spin-label or EDTA-Cu2+ tags as a model. By …

Metal ions based dynamic nuclear polarization: MI-DNP

D Jardón-Álvarez, M Leskes - Progress in Nuclear Magnetic Resonance …, 2023 - Elsevier
Over the last two decades magic angle spinning dynamic nuclear polarization (MAS DNP)
has revolutionized NMR for materials characterization, tackling its main limitation of …

Accurate electron–nucleus distances from paramagnetic relaxation enhancements

HW Orton, G Otting - Journal of the American Chemical Society, 2018 - ACS Publications
Measurements of paramagnetic relaxation enhancements (PREs) in 1H NMR spectra are an
important tool to obtain long-range distance information in proteins, but quantitative …

Exploiting heterogeneous time scale of dynamics to enhance 2D HETCOR solid-state NMR sensitivity

R Zhang, Y Nishiyama, A Ramamoorthy - Journal of Magnetic Resonance, 2019 - Elsevier
Multidimensional solid-state NMR spectroscopy plays a significant role in offering atomic-
level insights into molecular systems. In particular, heteronuclear chemical shift correlation …

Monitoring electron spin fluctuations with paramagnetic relaxation enhancement

D Jardón-Álvarez, T Malka, J van Tol, Y Feldman… - Journal of Magnetic …, 2022 - Elsevier
The magnetic interactions between the spin of an unpaired electron and the surrounding
nuclear spins can be exploited to gain structural information, to reduce nuclear relaxation …

[HTML][HTML] Lipid-nanodiscs formed by paramagnetic metal chelated polymer for fast NMR data acquisition

GM Di Mauro, NZ Hardin, A Ramamoorthy - Biochimica et Biophysica Acta …, 2020 - Elsevier
Lipid-nanodiscs have been shown to be an exciting innovation as a membrane-mimicking
system for studies on membrane proteins by a variety of biophysical techniques, including …