Biorefineries are an essential step towards implementing a circular economy in the long
term. They are based on renewable raw materials and must be designed holistically …

A major obstacle to the bioconversion of lignocellulosic biomass is its complex structure and
recalcitrance. Chimeric enzymes, by expressing different enzymatic reactions in a single …

The well-known probiotic GRAS Saccharomyces boulardii (CNCM I-745) was used for the
first time to produce glutathione (GSH). The culture conditions affecting GSH biosynthesis …

Glutathione, a tri-peptide (glutamate-cysteine-glycine) with the thiol group (-SH), is most
efficient antioxidative agent in eukaryotic cells. The present study aimed to isolate an …

A β-mannanase gene was cloned from Bacillus sp. MK-2 and expressed in Bacillus subtilis
WB800. The ORF of the β-mannanase gene was 1104 bp in length, encoding 367 aa. The …

A rational design method to improve β-mannanase (ManTJ102) thermostability was
developed successfully in this study. The flexible area of residues 330–340 in ManTJ102 …

Background Synthetic biology is an emerging multidisciplinary area of research with the
potential to deliver various novel agrifood applications. Its long-term adoption and …

To study the molecular basis for thermophilic β-mannanase of glycoside hydrolase family 5,
two β-mannanases, Tl Man5A and P Man5A, from Talaromyces leycettanus JCM12802 and …

Large amounts of spent coffee grounds are produced daily and represent a global
agricultural waste issue. Therefore, conversion of spent coffee grounds to fine chemicals by …

Glutathione has diverse physiological functions, and therefore, the demand for it has
increased recently. Currently, industrial mass production of glutathione is performed from D …