Until recently, class A penicillin‐binding proteins (aPBPs) were the only enzymes known to
catalyze glycan chain polymerization from lipid II in bacteria. Hence, the discovery of two …

The rise in fluorescence-based imaging techniques over the past 3 decades has improved
the ability of researchers to scrutinize live cell biology at increased spatial and temporal …

Penicillin-binding proteins (PBPs) are key to the assembly of peptidoglycan, the major
component of the bacterial cell wall. Although several PBP-specific regulatory proteins have …

ABSTRACT MOP (Multidrug/Oligosaccharidyl-lipid/Polysaccharide) family transporters are
found in almost all life forms. They are responsible for transporting lipid-linked precursors …

Coordinated membrane and cell wall synthesis is vital for maintaining cell integrity and
facilitating cell division in bacteria. However, the molecular mechanisms that underpin such …

Control of peptidoglycan assembly is critical to maintain bacterial cell size and morphology.
Penicillin-binding proteins (PBPs) are crucial enzymes for the polymerization of the glycan …

The peptidoglycan (PG) layer protects bacteria from osmotic lysis and defines their shape.
The class A penicillin-binding proteins (aPBPs) are PG synthases that possess both glycan …

RNases perform indispensable functions in regulating gene expression in many bacterial
pathogens by processing and/or degrading RNAs. Despite the pivotal role of RNases in …

Transposon insertion sequencing (TIS) is a widely used technique for conducting genome-
scale forward genetic screens in bacteria. However, few methods enable comparison of TIS …

Penicillin‐binding proteins (PBPs) are crucial enzymes of peptidoglycan assembly and
targets of β‐lactam antibiotics. However, little is known about their regulation. Recently …